Various lysates were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 15417-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15417-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15417-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 15417-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
COLO 320 cells were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 15417-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
rat liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15417-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HT-29 using 15417-1-AP
HT-29 cells were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HT-29 cells were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 15417-1-AP
IP result of anti-BDH1 (IP:15417-1-AP, 4ug; Detection:15417-1-AP 1:300) with mouse liver tissue lysate 4000ug.
IP result of anti-BDH1 (IP:15417-1-AP, 4ug; Detection:15417-1-AP 1:300) with mouse liver tissue lysate 4000ug.
IHC staining of rat brain using 15417-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15417-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15417-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse liver using 15417-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using BDH1 antibody (15417-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HepG2 cells, mouse liver tissue, rat liver tissue, HT-29 cells, human liver tissue, rat brain tissue, COLO 320 cells, mouse brain tissue, mouse heart tissue
Positive IP detected in
mouse liver tissue
Positive IHC detected in
rat brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse liver tissue
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).
Various lysates were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 15417-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15417-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15417-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 15417-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 15417-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15417-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HT-29 using 15417-1-AP
HT-29 cells were subjected to SDS PAGE followed by western blot with 15417-1-AP (BDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of rat brain using 15417-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15417-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15417-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15417-1-AP (BDH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse liver using 15417-1-AP
IP result of anti-BDH1 (IP:15417-1-AP, 4ug; Detection:15417-1-AP 1:300) with mouse liver tissue lysate 4000ug.
IF-P Figures
IF Staining of mouse liver using 15417-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using BDH1 antibody (15417-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
BDH1 Polyclonal antibody
Catalog Number
15417-1-AP
Citations
27
Dilutions
WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF-P : 1:50-1:500
Applications
WB, IHC, IF-P, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
