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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HSC-T6 cells, Jurkat cells, K-562 cells, Raji cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
66762-1-Ig targets BACH1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Rat, mouse, pig, rabbit samples.
| Tested Reactivity | Human, Rat, mouse, pig, rabbit |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BACH1 fusion protein Ag6011 Predict reactive species |
| Full Name | BTB and CNC homology 1, basic leucine zipper transcription factor 1 |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC063307 |
| Gene Symbol | BACH1 |
| Gene ID (NCBI) | 571 |
| ENSEMBL Gene ID | ENSG00000156273 |
| RRID | AB_2882108 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O14867 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BACH1 antibody 66762-1-Ig | Download protocol |
| IHC protocol for BACH1 antibody 66762-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism
| ||
Life Sci Alliance A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95 | ||
Cancer Sci BACH1 promotes clear cell renal cell carcinoma progression by upregulating oxidative stress-related tumorigenicity
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