Various lysates were subjected to SDS PAGE followed by western blot with 25727-1-AP (ATP1A3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 25727-1-AP (ATP1A3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of rat brain using 25727-1-AP
IP result of anti-ATP1A3 (IP:25727-1-AP, 4ug; Detection:25727-1-AP 1:500) with rat brain tissue lysate 4000ug.
IP result of anti-ATP1A3 (IP:25727-1-AP, 4ug; Detection:25727-1-AP 1:500) with rat brain tissue lysate 4000ug.
IHC staining of human prostate cancer using 25727-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25727-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25727-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 25727-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25727-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25727-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 25727-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ATP1A3 antibody (25727-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ATP1A3 antibody (25727-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 25727-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ATP1A3 antibody (25727-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ATP1A3 antibody (25727-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse brain tissue
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:16000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na/K homeostasis. This antibody is generated against the C-terminal region (665-1013aa) of ATP1A3 and detects the band around 100-113 kDa in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)
Various lysates were subjected to SDS PAGE followed by western blot with 25727-1-AP (ATP1A3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human prostate cancer using 25727-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25727-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 25727-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25727-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of rat brain using 25727-1-AP
IP result of anti-ATP1A3 (IP:25727-1-AP, 4ug; Detection:25727-1-AP 1:500) with rat brain tissue lysate 4000ug.
IF-P Figures
IF Staining of mouse brain using 25727-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ATP1A3 antibody (25727-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 25727-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ATP1A3 antibody (25727-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ATP1A3 Polyclonal antibody
Catalog Number
25727-1-AP
Citations
2
Dilutions
WB : 1:2000-1:16000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF-P : 1:50-1:500
Applications
WB, IHC, IF-P, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
