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PARK9/ATP13A2 Polyclonal antibody

PARK9/ATP13A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19141-1-AP

Synonyms

ATP13A2, ATPase type 13A2, HSA9947, KRPPD, PARK9, PARK9/ATP13A2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PARK9/ATP13A2 Polyclonal antibody (19141-1-AP)

    WB analysis of mouse brain using 19141-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19141-1-AP (PARK9/ATP13A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PARK9/ATP13A2 Polyclonal antibody (19141-1-AP)

    IHC staining of mouse brain using 19141-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19141-1-AP (PARK9/ATP13A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PARK9/ATP13A2 Polyclonal antibody (19141-1-AP)

    IHC staining of mouse brain using 19141-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19141-1-AP (PARK9/ATP13A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19141-1-AP targets PARK9/ATP13A2 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARK9/ATP13A2 fusion protein Ag11435
Full Name ATPase type 13A2
Calculated Molecular Weight 1158 aa, 126 kDa
Observed Molecular Weight125-130 kDa
GenBank Accession NumberBC030267
Gene Symbol ATP13A2
Gene ID (NCBI) 23400
RRIDAB_2918064
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP13A2 belongs to the P-type superfamily of ATPases that transport inorganic cations and other substrates across cell membranes. It appears to protect cells from manganese and zinc toxicity, possibly by causing cellular efflux and/or lysosomal sequestration; and from iron toxicity, possibly by preserving lysosome integrity against iron-induced lipid peroxidation (21724849)(24334770). However, it potentiates the toxic effects of cadmium and nickel on developing neurites, and of the widely used herbicide paraquat possibly by increasing polyamine uptake. Deficiency is associated with spastic paraplegia and Kufor-Rakeb syndrome, in which there is progressive parkinsonism with dementia.

Protocols

Product Specific Protocols
WB protocol for PARK9/ATP13A2 antibody 19141-1-APDownload protocol
IHC protocol for PARK9/ATP13A2 antibody 19141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols