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WB analysis using 20380-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 20380-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 20380-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 20380-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20380-1-AP (AQP9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 20380-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20380-1-AP (AQP9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 20380-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse testis using 20380-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human liver using 20380-1-AP
Immunofluorescent analysis of (4% PFA) fixed human liver tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human liver using 20380-1-AP
Immunofluorescent analysis of (4% PFA) fixed human liver tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse testis using 20380-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

AQP9 Polyclonal antibody

Name: AQP9 Polyclonal antibody
Brand: Proteintech
SKU: 20380-1-AP

AQP9 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 20380-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

HsT17287, Aquaporin-9, Aquaglyceroporin-9, Aquaglyceroporin 9, AQP-9

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	L02 cells, mouse brain tissue, rat brain tissue, mouse liver tissue, mouse lung tissue, mouse testis tissue, rat liver tissue, rat testis tissue
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human liver tissue, mouse brain tissue, mouse testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20380-1-AP targets AQP9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AQP9 fusion protein Ag14200 Predict reactive species
Full Name	aquaporin 9
Calculated Molecular Weight	295 aa, 31 kDa
Observed Molecular Weight	31 kDa
GenBank Accession Number	BC026258
Gene Symbol	AQP9
Gene ID (NCBI)	366
RRID	AB_2935448
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O43315
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aquaporins (AQPs), also known as water channel proteins, are members of a large protein family that osmotically modulate water fluid homeostasis in several tissues. AQP9 (Aquaporin 9) is a member of the aquaporin family that acts as a water-selective membrane channel, and it may be involved in cell migration, angiogenesis, and tumor growth. AQP9 is involved in arsenic uptake in hepatocellular carcinoma cells, in which p38-MAPK may play a limited role. AQP9 was demonstrated to promote astrocytoma cell invasion and motility via the AKT pathway.

Protocols

Product Specific Protocols
WB protocol for AQP9 antibody 20380-1-AP	Download protocol
IHC protocol for AQP9 antibody 20380-1-AP	Download protocol
IF protocol for AQP9 antibody 20380-1-AP	Download protocol