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  • Featured Product
  • KD/KO Validated

Annexin A11 Polyclonal antibody

Annexin A11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10479-2-AP

Synonyms

ANXA11, ANX11, Annexin-11, Annexin XI, Annexin 11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin A11 Polyclonal antibody (10479-2-AP)

    WB analysis using 10479-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10479-2-AP (Annexin A11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Annexin A11 Polyclonal antibody (10479-2-AP)

    WB analysis of HeLa using 10479-2-AP

    HeLa (WT and ANXA11 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 10479-2-AP incubated at 1:2000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Immunoprecipitation (IP) experiment of HeLa cells using Annexin A11 Polyclonal antibody (10479-2-AP)

    IP experiment of HeLa using 10479-2-AP

    HeLa lysates prepared and IP of ANXA11 performed using 1.0 μg of 10479-2-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Immunoprecipitation (IP) experiment of HeLa cells using Annexin A11 Polyclonal antibody (10479-2-AP)

    IP experiment of HeLa using 10479-2-AP

    IP result of anti-Annexin A11 (IP:10479-2-AP, 4ug; Detection:10479-2-AP 1:1000) with HeLa cells lysate 2320 ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Annexin A11 Polyclonal antibody (10479-2-AP)

    IHC staining of human ovary tumor using 10479-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Annexin A11 Polyclonal antibody (10479-2-AP)

    IHC staining of human ovary tumor using 10479-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Annexin A11 Polyclonal antibody (10479-2-AP)

    IHC staining of human kidney using 10479-2-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10479-2-AP (Annexin A11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Annexin A11 Polyclonal antibody (10479-2-AP)

    IHC staining of human kidney using 10479-2-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10479-2-AP (Annexin A11 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Annexin A11 Polyclonal antibody (10479-2-AP)

    IHC staining of human breast cancer using 10479-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Annexin A11 Polyclonal antibody (10479-2-AP)

    IHC staining of human breast cancer using 10479-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Annexin A11 Polyclonal antibody (10479-2-AP)

    IHC staining of human lung cancer using 10479-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Annexin A11 Polyclonal antibody (10479-2-AP)

    IHC staining of human lung cancer using 10479-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin A11 Polyclonal antibody (10479-2-AP)

    IF Staining of HeLa using 10479-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Annexin A11 antibody (10479-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin A11 Polyclonal antibody (10479-2-AP)

    IF Staining of HeLa using 10479-2-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (10479-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin A11 Polyclonal antibody (10479-2-AP)

    IF Staining of HeLa using 10479-2-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (10479-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, HeLa cells, mouse uterus tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human kidney tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10479-2-AP targets Annexin A11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A11 fusion protein Ag0729
Full Name annexin A11
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56-60 kDa
GenBank Accession NumberBC007564
Gene Symbol Annexin A11
Gene ID (NCBI) 311
RRIDAB_2057171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.

Protocols

Product Specific Protocols
WB protocol for Annexin A11 antibody 10479-2-APDownload protocol
IHC protocol for Annexin A11 antibody 10479-2-APDownload protocol
IF protocol for Annexin A11 antibody 10479-2-APDownload protocol
IP protocol for Annexin A11 antibody 10479-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Sci Transl Med

Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis.

Authors - Bradley N Smith
View Article
humanIHC,IF

Ann Neurol

A Novel Multisystem Proteinopathy Caused by a Missense ANXA11 Variant.

Authors - Tauana Bernardes Leoni
View Article
mouseWB

Oncotarget

ANXA11 regulates the tumorigenesis, lymph node metastasis and 5-fluorouracil sensitivity of murine hepatocarcinoma Hca-P cells by targeting c-Jun.

Authors - Shuqing Liu
  • KD Validated
View Article
mouseWB

Biomed Pharmacother

Annexin A11 knockdown inhibits in vitro proliferation and enhances survival of Hca-F cell via Akt2/FoxO1 pathway and MMP-9 expression.

Authors - Shuqing Liu
  • KD Validated
View Article
humanWB,IHC

Neurobiol Aging

Genetic screening of ANXA11 revealed novel mutations linked to amyotrophic lateral sclerosis.

Authors - Elisa Teyssou
View Article
humanIHC

Ann Clin Transl Neurol

Adult-onset dominant muscular dystrophy in Greek families caused by Annexin A11

Authors - Mridul Johari
View Article