Multiplex Immunofluorescence (mIF): How to Image the Rainbow
Multiplex immunofluorescence (mIF) is a powerful tool that allows for the simultaneous detection of multiple antigens on a single tissue section and is ideal to study complex spatial biology. Starting a mIF experiment can be intimidating, especially when using multiple antibodies from the same species.
In this mIF workshop, you'll learn more about:
- IF and mIF workflows
- How to select the correct dyes, antibodies, and other reagents
- Tips and tricks for your mIF workflow
- Common obstacles and troubleshooting
Workshop led by Derek Sung, MD/PhD candidate at the University of Pennsylvania Perelman School of Medicine