Various lysates were subjected to SDS PAGE followed by western blot with 16644-1-AP (TIMP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using TIMP1 antibody (16644-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (83151-5-RR, Clone: 230329E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TIMP1 antibody (83151-5-RR, Clone: 230329E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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1x10^6 HT-29 cells were intracellularly stained with 0.25 ug TIMP1 Recombinant antibody (83151-5-RR, Clone:230329E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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1x10^6 MDA-MB-231 cells were intracellularly stained with 0.25 ug TIMP1 Recombinant antibody (83151-5-RR, Clone:230329E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Standard curve of Human TIMP1 ELISA Kit KE00166.

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Standard curve of Rat TIMP1 ELISA Kit KE20004.

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Sandwich ELISA standard curve of MP50084-1, TIMP1 Monoclonal Matched Antibody Pair - PBS only. 68691-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0638. 68691-2-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

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Sandwich ELISA standard curve of MP50084-2, TIMP1 Monoclonal Matched Antibody Pair - PBS only. 68691-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0638. 68691-4-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

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Sandwich ELISA standard curve of MP50084-2, TIMP1 Monoclonal Matched Antibody Pair - PBS only. 68691-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0638. 68691-4-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

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