Purity of recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Recombinant human TGF beta 1 (HZ-1011) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.01-0.17 ng/mL.
 

Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)

Purity of GMP recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Recombinant human GMP TGF beta 1 (HZ-1011-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant GMP TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.01-0.17 ng/mL.

Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

Indirect ELISA was carried out by coating recombinant Human TGF beta 1 (Cat.NO. HZ-1011) at 70 ng/well followed by blocking and adding serial diluted TGF beta 1 antibody 69012-1-Ig and 69512-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 69012-1-Ig (NeutraKine® TGF beta 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 69012-1-Ig (NeutraKine® TGF beta 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0131 (TGF beta 1 IHC Kit).

Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

Indirect ELISA was carried out by coating recombinant Human TGF beta 1 (Cat.NO. HZ-1011) at 70 ng/well followed by blocking and adding serial diluted TGF beta 1 antibody 69012-1-Ig and 69512-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

Various lysates were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TGF beta 1 antibody (26155-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).