Various lysates were subjected to SDS PAGE followed by western blot with 13161-1-AP (SIRT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (13161-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13161-1-AP (SIRT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (13161-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

WB result of SIRT1antibody (60303-1-Ig, 1:500) with si-Control and si-SIRT1 transfected HEK-293 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

IP result of anti-SIRT1 (IP:60303-1-Ig, 5ug; Detection:60303-1-Ig 1:1000) with HeLa cells lysate 2000ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60303-1-Ig(SIRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with CL750-13161 (SIRT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 750 Anti-Human SIRT1 (CL750-13161) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC1207 (SIRT1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using KHC1207 (SIRT1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using KHC1207 (SIRT1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using KHC1207 (SIRT1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using KHC1207 (SIRT1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using KHC1207 (SIRT1 IHC Kit).

Various lysates were subjected to SDS PAGE followed by western blot with 82790-4-RR (SIRT1 antibody) at dilution of 1:4700 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SIRT1 antibody (82790-4-RR, Clone: 2F11 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

Various lysates were subjected to SDS PAGE followed by western blot with 82790-4-RR (SIRT1 antibody) at dilution of 1:4700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82790-4-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SIRT1 antibody (82790-4-RR, Clone: 2F11 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82790-4-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

WB result of SIRT1antibody (60303-1-Ig, 1:500) with si-Control and si-SIRT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

IP result of anti-SIRT1 (IP:60303-1-Ig, 5ug; Detection:60303-1-Ig 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60303-1-Ig(SIRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 82790-2-RR (SIRT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.