Serial dilutions of HeLa cell lysate Primary: Proteintech, Beta Catenin (51067-2-AP), 1:20,000 <br>Secondary: Quanta BioDesign HRP-Goat anti-Rabbit IgG (H&L) (11-0201-0503) 1:100,000<br>Exposure Time: 30 seconds<br>SignalBright Max Chemiluminescent substrate
Serial dilutions of HeLa cell lysate <br>Primary: Proteintech, Beta-actin (66009-1-Ig); 1:100,000<br>Secondary: Quanta BioDesign HRP-Goat anti-Mouse IgG (H&L) (11-0101-0303) 1:75,000<br>Exposure Time: 120 seconds<br>SignalBright Max Chemiluminescent substrate