Serial dilutions of HeLa cell lysate Primary: Proteintech, Beta Catenin  (51067-2-AP), 1:20,000 <br>Secondary: Quanta BioDesign HRP-Goat anti-Rabbit IgG (H&L) (11-0201-0503) 1:100,000<br>Exposure Time: 30 seconds<br>SignalBright Max Chemiluminescent substrate

---

Serial dilutions of HeLa cell lysate <br>Primary: Proteintech, Beta-actin (66009-1-Ig); 1:100,000<br>Secondary: Quanta BioDesign HRP-Goat anti-Mouse IgG (H&L) (11-0101-0303) 1:75,000<br>Exposure Time: 120 seconds<br>SignalBright Max Chemiluminescent substrate

---

Serial dilutions of HeLa cell lysate <br>Primary: Proteintech, beta-actin 66009-1-Ig; 1:50,000<br>Secondary: Quanta BioDesign HRP-Goat anti-Mouse IgG (H&L) (11-0101-0303) 1:60,000<br>Exposure Time: 30 seconds<br>SignalBright Plus Chemiluminescent substrate

---

Serial dilutions of HeLa cell lysate <br>Primary: Proteintech Beta Catenin  (51067-2-AP), 1:10,000 <br>Secondary: Quanta BioDesign HRP-Goat anti-Rabbit IgG (H&L) (11-0201-0503); 1:50,000<br>Exposure Time: 40 seconds<br>SignalBright Plus Chemiluminescent substrate

---

Serial dilutions of Hela cell lysates<br>Primary: Proteintech Proteintech, beta-actin (66009-1-Ig); 1:50,000<br>Secondary: Quanta BioDesign HRP-Goat anti-Mouse IgG (H&L) (11-0101-0303) 1:50,000<br>Exposure Time: 30 seconds<br>SignalBright Pro Chemiluminescent substrate

---