ChromoTek SARS-CoV-2 Spike Recombinant VHH [NM1266]
Recombinant bivalent Nanobody against the Receptor-binding domain (RBD) of SARS- CoV-2 Spike protein
Specificity
Virus
Applications
BLI, Multiplex ACE2 competition assay
Conjugate
Unconjugated
Cat no : sc-NM1266
Synonyms
Validation Data Gallery
![BLI binding kinetics of SARS-CoV-2 Spike Recombinant VHH [NM1266] to RBD. Biotinylated RBD was immobilized on FortéBio Streptavidin (SA) Biosensors and assayed with 25 nM of SARS- CoV-2 Spike Recombinant VHH [NM1266] (ChromoTek sc-NM1266). For comparison the binding kinetics of 25 nM SARS-CoV-2 Spike Recombinant VHH [NM1228] (ChromoTek sc- NM1228) and SARS-CoV-2 Spike Recombinant VHH [NM1230] (ChromoTek sc-NM1230) are shown.](/products/pictures/Result-sc-NM1266.png "BLI binding kinetics of SARS-CoV-2 Spike Recombinant VHH [NM1266] to RBD. Biotinylated RBD was immobilized on FortéBio Streptavidin (SA) Biosensors and assayed with 25 nM of SARS- CoV-2 Spike Recombinant VHH [NM1266] (ChromoTek sc-NM1266). For comparison the binding kinetics of 25 nM SARS-CoV-2 Spike Recombinant VHH [NM1228] (ChromoTek sc- NM1228) and SARS-CoV-2 Spike Recombinant VHH [NM1230] (ChromoTek sc-NM1230) are shown.")
Product Information
Recombinant bivalent Nanobody against the Receptor-binding domain (RBD) of SARS- CoV-2 Spike protein
| Specificity | Virus |
| Clonality | Monoclonal |
| Host | Alpaca, recombinantly produced |
| Class | Recombinant |
| Clone No. | NM1266 (NM1230-NM1228 fusion) [Wagner et al. EMBO Rep. 2021 May 5;22(5):e52325. PMID: 33904225] |
| Type | Nanobody / VHH, biparatopic |
| Epitope | NM1230: Amino acids 351 and 437-503 (determined by X-Ray crystallography) NM1228: Amino acids 370-387 and 489-514 (determined by HDX-MS) |
| RRID | AB_2892256 |
| Immunogen | RBD of SARS-CoV-2 Spike protein |
| Full Name | SARS-CoV-2 Spike Protein |
| Calculated MW | 25 kDa |
| GenBank Accession Number | NC_045512 |
| Gene Symbol | COVID-19 S Protein |
| GeneID (NCBI) | 43740568 |
| Affinity (KD) | NM1230: 9.5 nM NM1228: 0.75 nM |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Recombinant expression, affinity purification by IMAC. Note: SARS-CoV-2 Spike Recombinant VHH carries a C-terminal His-tag. |
| Concentration | 1000 µg/mL (34.8 µM) |
| Positive Controls | BLI: biotinylated RBD and SARS-CoV-2 Spike Recombinant VHH |
| Cited Applications | BLI, Multiplex ACE2 competition assay Note: Not recommended for Western blotting |
| Storage Buffer | PBS, 0.09% sodium azide Safety datasheet (SDS): sodium azide |
| Storage Condition | Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles. |
| Stability | Stable for 1 year at -20°C/-4°F after shipment. |
| Shipment | Shipped at ambient temperature. |