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ChromoTek SARS-CoV-2 Spike Recombinant VHH [NM1221]

Recombinant monovalent Nanobody against the Receptor-binding domain (RBD) of SARS-CoV-2 Spike protein

Specificity

Virus

Applications

BLI, Multiplex ACE2 competition assay

Conjugate

Unconjugated

Cat no : sc-NM1221

Synonyms

SARS-CoV-2, SARS-CoV-2 Spike, Spike

Validation Data Gallery

  • BLI binding kinetics of SARS-CoV-2 Spike Recombinant VHH [NM1221] to RBD. Biotinylated RBD was immobilized on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of SARS-CoV-2 Spike Recombinant VHH [NM1221] (ChromoTek sc-NM1221).

    BLI binding kinetics of SARS-CoV-2 Spike Recombinant VHH [NM1221] to RBD. Biotinylated RBD was immobilized on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of SARS-CoV-2 Spike Recombinant VHH [NM1221] (ChromoTek sc-NM1221).

Product Information

Recombinant monovalent Nanobody against the Receptor-binding domain (RBD) of SARS-CoV-2 Spike protein

SpecificityVirus
HostAlpaca, recombinantly produced
ClonalityMonoclonal
Clone No.NM1221 [Wagner et al. EMBO Rep. 2021 May 5;22(5):e52325. PMID: 33904225]
ClassRecombinant
TypeNanobody / VHH, monovalent
EpitopeAmino acids 432-452 and 487-496 (determined by HDX-MS)
RRIDAB_2892247
ImmunogenRBD of SARS-CoV-2 Spike protein
Full NameSARS-CoV-2 Spike protein
Calculated MW25 kDa
GenBank Accession NumberNC_045512
Gene SymbolCOVID-19 S Protein
GeneID (NCBI)43740568
Affinity (KD) 27 nM
ConjugateUnconjugated
FormLiquid
Purification MethodRecombinant expression, affinity purification by IMAC.
Note: SARS-CoV-2 Spike Recombinant VHH carries a C-terminal Myc-tag and His-tag.
Concentration1000 µg/mL (65.1 µM)
Positive ControlsBLI: biotinylated RBD and SARS-CoV-2 Spike Recombinant VHH
Cited ApplicationsBLI, Multiplex ACE2 competition assay
Note: Not recommended for Western blotting
Storage BufferPBS, 0.09% sodium azide
Safety datasheet (SDS): sodium azide
Storage ConditionAliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles.
StabilityStable for 1 year at -20°C/-4°F after shipment.
ShipmentShipped at ambient temperature.