Various lysates were subjected to SDS PAGE followed by western blot with 82707-13-RR (Cleaved Caspase 3; P17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82707-13-RR (Cleaved Caspase 3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82707-13-RR (Cleaved Caspase 3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cleaved Caspase 3 antibody (82707-13-RR, Clone: 230527B2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

Various lysates were subjected to SDS PAGE followed by western blot with 82024-1-RR (Galectin 3 antibody) at dilution of 1:9900 incubated at room temperature for 1.5 hours.

WB result of Galectin 3 antibody (82024-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin 3 transfected HeLa cells.

IP result of anti-Galectin 3 (IP:82024-1-RR, 4ug; Detection:82024-1-RR 1:10000) with MCF-7 cells lysate 1880 ug.

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82024-1-RR (Galectin 3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Galectin 3 antibody (82024-1-RR, Clone: 4M10 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Biolayer interferometry (BLl) kinetic assays of 82024-1-RR against Human Galectin 3 were performed. The affinity constant is 1.17 nM.

Various lysates were subjected to SDS PAGE followed by western blot with 82707-13-RR (Cleaved Caspase 3; P17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82707-13-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82707-13-RR (Cleaved Caspase 3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82707-13-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82707-13-RR (Cleaved Caspase 3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82707-13-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cleaved Caspase 3 antibody (82707-13-RR, Clone: 230527B2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82707-13-PBS in a different storage buffer formulation.

Cytometric bead array standard curve of MP00104-1, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-9-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Cytometric bead array standard curve of MP00104-2, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Cytometric bead array standard curve of MP00104-3, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-8-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-2.5 ng/mL.

Cytometric bead array standard curve of MP00104-1, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-9-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Cytometric bead array standard curve of MP00104-2, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Cytometric bead array standard curve of MP00104-3, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-8-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-2.5 ng/mL.

Cytometric bead array in cell lysate using MP00589-1

Cytometric bead array in cell lysate using MP00589-2

1x10^6 human PBMCs were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red in histogram), or 0.2 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (green in histogram), or unstained (blue in histogram). Cells were not fixed.

IP result of anti-IgG Isotype Control (IP:98136-1-RR, 4ug; Detection:98136-1-RR 1:0) with HepG2 cells lysate 2000 ug.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). NOL11 Recombinant antibody (83391-6-RR) is a positive control.

1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug PGF Recombinant antibody (83170-4-RR, Clone:230532H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

HeLa cells were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 83793-5-RR (Cadherin-17 Recombinant antibody) or unstained (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Sample test of MP00550-1

Cytometric bead array standard curve of MP00550-1

Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Various lysates were subjected to SDS PAGE followed by western blot with 80762-1-RR (Tubulin-Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

WB result of Alpha Tubulin antibody (80762-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HEK-293 cells.

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Biolayer interferometry (BLl) kinetic assays of 80762-1-RR against Human Alpha Tubulin were performed. The affinity constant is 0.708 nM.

Various lysates were subjected to SDS PAGE followed by western blot with 82724-1-RR (LDLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDLR antibody (82724-1-RR, Clone: 1O11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Biolayer interferometry (BLl) kinetic assays of 82724-1-RR against Human LDLR were performed. The affinity constant is below 1 pM.

Various lysates were subjected to SDS PAGE followed by western blot with 81323-2-RR (VEGFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VEGFA antibody (81323-2-RR, Clone: 7H10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Biolayer interferometry (BLl) kinetic assays of 81323-2-RR against Human VEGFA were performed. The affinity constant is 1.60 nM.

Various lysates were subjected to SDS PAGE followed by western blot with 81640-5-RR (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GAPDH antibody (81640-5-RR, Clone: 1H18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Biolayer interferometry (BLl) kinetic assays of 81640-5-RR against Human GAPDH were performed. The affinity constant is 10.4 nM.

Various lysates were subjected to SDS PAGE followed by western blot with 81640-5-RR (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81640-5-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GAPDH antibody (81640-5-RR, Clone: 1H18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 81640-5-PBS in a different storage buffer formulation.

Cytometric bead array standard curve of MP00549-1, GAPDH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81640-3-PBS. Detection antibody: 81640-5-PBS. Standard:Ag0766. Range: 0.098-100 ng/mL.

Biolayer interferometry (BLl) kinetic assays of 81640-5-RR against Human GAPDH were performed. The affinity constant is 10.4 nM.

Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-Beta Tubulin (IP:80713-1-RR, 4ug; Detection:80713-1-RR 1:10000) with HEK-293 cells lysate 1610 ug.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug Beta Tubulin Recombinant antibody (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Biolayer interferometry (BLl) kinetic assays of 80713-1-RR against Human Beta Tubulin were performed. The affinity constant is below 1 pM.

1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD45 Rabbit Recombinant Antibody (98117-1-RR, Clone: 241670E10) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (98117-1-RR, Clone: 241670E10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Biolayer interferometry (BLl) kinetic assays of 98117-1-RR against Human CD45 were performed. The affinity constant is 2.58 nM.

1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD45 Rabbit Recombinant Antibody (98117-1-RR, Clone: 241670E10) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98117-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (98117-1-RR, Clone: 241670E10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 98117-1-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 98117-1-RR against Human CD45 were performed. The affinity constant is 2.58 nM.

Various lysates were subjected to SDS PAGE followed by western blot with 80912-4-RR (OPN, SPP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

1x10^6 HepG2 cells were intracellularly stained with 0.25 ug OPN, SPP1 Recombinant antibody (80912-4-RR, Clone:6B11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Biolayer interferometry (BLl) kinetic assays of 80912-4-RR against Human OPN, SPP1 were performed. The affinity constant is 199 nM.

Various lysates were subjected to SDS PAGE followed by western blot with 83961-4-RR (Adiponectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Biolayer interferometry (BLl) kinetic assays of 83961-4-RR against Human ADIPOQ were performed. The affinity constant is below 1 pM.