Untreated and MG132 treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Untreated and MG132 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Untreated and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-NRF2, NFE2L2 (IP:80593-1-RR, 4ug; Detection:80593-1-RR 1:700) with HeLa cells lysate 1520 ug.
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NRF2, NFE2L2 antibody (80593-1-RR, Clone: 1I21 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using NRF2, NFE2L2 antibody (80593-1-RR, Clone: 1I21 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Biolayer interferometry (BLl) kinetic assays of 80593-1-RR against Human NRF2/NFE2L2 were performed. The affinity constant is 0.191 nM.