Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human TNF alpha (HZ-1014) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL.
 

Proteintech TNF alpha (HZ-1014) demonstrates equivalent induction cytotoxicity and EC50 to leading competitors. TNF alpha demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL.

Purity of recombinant human IL-1 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

ecombinant human IL-1 beta (HZ-1164) stimulates does-dependent proliferation of the D10.G4.1 mouse helper t lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. D10.G4.1 cells were treated with increasing concentrations of recombinant IL-1 beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. 
 

Recombinant human IL-1 beta (Cat no: HZ-1164) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.8-4.0 ng/mL ng/mL EC50.
 

Purity of recombinant human MCP-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human MCP-1 (HZ-1334) stimulates dose-dependent proliferation of THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant MCP-1 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 150-750 ng/mL.

Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human TNF alpha (HZ-1014-GMP) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.002-0.026 ng/mL.

Various lysates were subjected to SDS PAGE followed by western blot with 25128-1-AP (cleaved Caspase 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25128-1-AP (Cleaved Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cleaved Caspase 3 antibody (25128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Cytometric bead array standard curve of MP00104-3, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-8-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-2.5 ng/mL.

Cytometric bead array standard curve of MP00104-1, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-9-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Cytometric bead array standard curve of MP00104-2, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Cytometric bead array standard curve of MP00104-2, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Cytometric bead array standard curve of MP00104-3, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-8-PBS. Detection antibody: 82707-11-PBS. Standard: SY01603. Range: 0.156-2.5 ng/mL.

Cytometric bead array standard curve of MP00104-1, Cleaved Caspase 3/P17 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82707-10-PBS. Detection antibody: 82707-9-PBS. Standard: SY01603. Range: 0.156-10 ng/mL.

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1 Antibody) at dilution of 1:200 (under 40x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/p20/p10 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/p20/p10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/p20/p10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

WB result of Caspase 1/p20/p10 antibody (22915-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 1/p20/p10 transfected HepG2 cells.

IF result of 22915-1-AP from publication with PMID: 31611559.

Non-treated THP-1 cells, THP-1 cells (treated with 100 ng/ml LPS for 3h, then added 300 ng/ml protein transport inhibitor for 3h) were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL-1 Beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 27458-1-AP (NLRP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-NLRP3 (IP:27458-1-AP, 4ug; Detection:27458-1-AP 1:800) with THP-1 cells lysate 1520 ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27458-1-AP (NLRP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27458-1-AP (NLRP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NLRP3 antibody (27458-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

mouse liver tissue were subjected to SDS PAGE followed by western blot with 19811-1-AP (TLR4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 40x lens).