Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28733-1-AP (ERK1/2-phospho-Thr202/Tyr204) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

IP result of anti-Phospho-ERK1/2 (Thr202/Tyr204) (IP:28733-1-AP, 2ug; Detection:28733-1-AP 1:1000) with Calyculin A treated PC-3 cells lysate 1552 ug.

Non-treated and PMA treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28733-1-AP (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Non-treated and Calyculin A treated HeLa cellls were subjected to SDS PAGE followed by western blot with 22309-1-AP (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours.

Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed LPS treated THP-1 cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody (28796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody (28796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28731-1-AP (Phospho-AKT (Ser473) antibody) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28731-1-AP (Phospho-AKT (Ser473) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.