Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14075-1-AP (PDGFD antibody) at dilution of 1:50 (under 10x lens).

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Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14075-1-AP (PDGFD antibody) at dilution of 1:50 (under 40x lens).

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Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PDGFD antibody (14075-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PDGFD antibody (14075-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Various lysates were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55076-1-AP (PDGFC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Recombinant human PDGFbb (Cat.NO. HZ-1308) stimulates proliferation of BALB/3T3 (mouse fibroblast cell line) in a dose dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGFbb (20ng/mL HZ-1308) is neutralized by mouse anti-human PDGFbb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL.

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Recombinant human PDGF bb (Cat.NO. HZ-1308) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGF bb (20 ng/mL HZ-1308) is neutralized by mouse anti-human PDGF bb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL. The NeutraControl mouse anti-human PDGF bb monoclonal antibody 69520-1-Ig could recognize human PDGF bb, but could not neutralize human PDGF bb at the range of 3.125-200 μg/mL(green curve, refer to top X-right Y axis).

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Indirect ELISA was carried out by coating recombinant Human PDGF bb (Cat.NO. HZ-1308) at 70 ng/well followed by blocking and adding serial diluted PDGF bb antibody 69020-1-Ig and 69520-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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Recombinant human PDGF bb (Cat.NO. HZ-1308) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGF bb (20 ng/mL HZ-1308) is neutralized by mouse anti-human PDGF bb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL. The NeutraControl mouse anti-human PDGF bb monoclonal antibody 69520-1-Ig could recognize human PDGF bb, but could not neutralize human PDGF bb at the range of 3.125-200 μg/mL(green curve, refer to top X-right Y axis).

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Indirect ELISA was carried out by coating recombinant Human PDGF bb (Cat.NO. HZ-1308) at 70 ng/well followed by blocking and adding serial diluted PDGF bb antibody 69020-1-Ig and 69520-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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Standard curve of Human PDGF-AB ELISA Kit KE00222.

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Various lysates were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83214-5-PBS in a different storage buffer formulation.

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Cytometric bead array standard curve of MP00198-2, PDGFB Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83214-4-PBS. Detection antibody: 83214-3-PBS. Standard: Ag25986. Range: 0.625-20 ng/mL.

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