Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18106-1-AP (PD-1/CD279 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18106-1-AP (PD-1/CD279 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 rabbit polyclonal antibody) at dilution of 1:100 and 60271-1-Ig (CD20 mouse monoclonal antibody) at dilution of 1:200, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 18106-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60271-1-Ig.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 rabbit polyclonal antibody) at dilution of 1:100 and 60271-1-Ig (CD20 mouse monoclonal antibody) at dilution of 1:200, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 18106-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60271-1-Ig.

Standard curve of Human PD1 ELISA Kit KE00075.

ELISA test of 65288-1-Ig. Mouse PD-1 (Eg0918) was coated at 70 ng/well followed by blocking. Serial diluted Rat anti-Mouse PD-1 antibody 65288-1-Ig was appied and detected with HRP conjugated secondary antibody. Signal was developed by TMB substrate.

Mouse PD-L1 (Eg0985) was coated at 70 ng/well. Serial diluted HRP conjugated mouse PD-1 (Eg0918) was added for binding test (blue cureve, refer to bottom X - left Y). Blocking test was performed by mixing serial diluted Rat anti-mouse PD-1 antibody 65288-1-Ig with 1 ug/mL HRP conjugated mouse PD-1 (Eg0918) followed by same dose coated Mouse PD-L1 (Eg0985) . Typicall ND50 is around 1ug/mL.

Mouse PD-1 (Eg0918) was coated at 70 ng/well. Serial diluted HRP conjugated mouse PD-L1 (Eg0985) was added for binding test (blue cureve, refer to bottom X - left Y). Blocking test was performed by mixing serial diluted Rat anti-mouse PD-1 antibody 65288-1-Ig with 1 ug/mL HRP conjugated mouse PD-L1 (Eg0985) followed by applyting to 70 ng/well coated Mouse PD-1 (Eg0918) . Typicall ND50 is around 1-5 ug/mL.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using KHC0064 (PD-1 IHC Kit).

Standard Curve of KE10121 Mouse PD-1 ELISA Kit

Various lysates were subjected to SDS PAGE followed by western blot with 29780-1-AP (PD-1/CD279 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 31445-1-AP (PD-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Purity of Recombinant Human PD-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Immobilized Human PD-1 (His tag) at 2 μg/mL (100 μL/well) can bind Human PD-L1 (hFc tag) with a linear range of 11-43 ng/mL.

HeLa cells were subjected to SDS PAGE followed by western blot with 14121-1-AP (RPL17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

IP result of anti-RPL17 (IP:14121-1-AP, 4ug; Detection:14121-1-AP 1:2000) with HeLa cells lysate 1080 ug.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14121-1-AP (RPL17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).