Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IF result of anti-p21 (CDKN1A, 10355-1-AP) in the myotome of a somite at E11.5 by Dr. Zalc A. and Dr. Relaix F. (Red = p21; Green=Pax3 from a GFP reporter; Blue=DAPI).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP (P21;CDKN1A Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP (P21;CDKN1A Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-P21 (IP:10355-1-AP, 4ug; Detection:10355-1-AP 1:2000) with MCF-7 cells lysate 1880 ug.

1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human P21 (10355-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 28248-1-AP (p21 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 67362-1-Ig (p21 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 P21 antibody (CL488-10355) at dilution of 1:100.

1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human P21 (CL488-10355) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human P21 (CL488-82669-2, Clone:3N14) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 P21 antibody (CL594-10355) at dilution of 1:100.

1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human P21 (CL594-10355) (red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 67362-1-Ig (p21 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67362-1-PBS in a different storage buffer formulation.