Results

GFP-Traps

5661 Publications

Reviews (32)

Applications

ChIP, CoIP, IP, RIP

Conjugation

Agarose, Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads), Magnetic agarose, Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads), Magnetic Particles M-270
-_- / -_-

Cat No. gtak-iST, gtd, gta, gtak, gtdk, gtma, gtmak, gtp, tbta, tbtak, tbtak-iST, tbtma, tbtmak
GFP-Traps (13 total products)
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RFP-Traps

406 Publications

Reviews (4)

Applications

ChIP, CoIP, IP, RIP

Conjugation

Agarose, Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads), Magnetic agarose, Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads), Magnetic Particles M-270
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Cat No. rta, rtak, rtak-iST, rtd, rtdk, rtma, rtmak
RFP-Traps (7 total products)
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Myc-Traps

266 Publications

Reviews (4)

Applications

IP

Conjugation

Agarose, Magnetic agarose
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Cat No. yta, ytak, ytak-iST, ytma, ytmak
Myc-Traps (5 total products)
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V5-Traps

38 Publications

Reviews (6)

Applications

ChIP, CoIP, IP, RIP

Conjugation

Agarose, Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads), Magnetic agarose, Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads), Magnetic Particles M-270
-_- / -_-

Cat No. v5ta, v5tak, v5tak-iST, v5td, v5tdk, v5tma, v5tmak
V5-Traps (7 total products)
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mNeonGreen-Traps

35 Publications

Applications

IP

Conjugation

Agarose, Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads), Magnetic agarose
-_- / -_-

Cat No. nta, ntak, ntak-iST, ntma, ntmak
mNeonGreen-Traps (5 total products)
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DYKDDDDK-Fab-Traps

33 Publications

Reviews (7)

Applications

ChIP, CoIP, IP, RIP

Conjugation

Agarose, Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
-_- / -_-

Cat No. ffa, ffak, ffap
DYKDDDDK-Fab-Traps (3 total products)
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MBP-Traps

19 Publications

Conjugation

Agarose
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Cat No. mbta, mbtak
MBP-Traps (2 total products)
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Spot-Traps

19 Publications

Reviews (1)

Applications

ChIP, CoIP, IP, RIP

Conjugation

Agarose, Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads), Magnetic agarose, Magnetic Particles M-270
-_- / -_-

Cat No. eta, etak, etak-iST, etd, etdk, etma, etmak
Spot-Traps (7 total products)
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GST-Traps

17 Publications

Conjugation

Agarose
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Cat No. sta, stak
GST-Traps (2 total products)
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Halo-Traps

16 Publications

Reviews (4)

Conjugation

Agarose, Magnetic agarose, Magnetic Particles M-270
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Cat No. ota, otak, otd, otdk, otma, otmak
Halo-Traps (6 total products)
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p53-N-term-Traps

9 Publications

Reviews (2)

Conjugation

Agarose
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Cat No. pta, ptak
p53-N-term-Traps (2 total products)
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PARP1-Traps

6 Publications

Conjugation

Agarose
-_- / -_-

Cat No. xta, xtak
PARP1-Traps (2 total products)
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p53-C-term-Traps

5 Publications

Conjugation

Agarose
-_- / -_-

Cat No. pta2, pta2k
p53-C-term-Traps (2 total products)
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SNAP-CLIP-tag-Traps

3 Publications

Conjugation

Agarose
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Cat No. wta, wtak
SNAP-CLIP-tag-Traps (2 total products)
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DNMT1-Traps

2 Publications

Conjugation

Agarose
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Cat No. dta, dtak
DNMT1-Traps (2 total products)
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MK2-Traps

2 Publications

Conjugation

Agarose
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Cat No. mta, mtak
MK2-Traps (2 total products)
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Ubiquitin-Traps

2 Publications

Reviews (1)

$The ubiquitin-trap agarose (uta) was used to immunoprecipitate endogenous ubiquitin and ubiquitinylated proteins from human (HEK293T), mouse (C2C12), and hamster (CHO) cell lines treated with MG-132. For each IP, samples of the input lysate (I), non-bound flow-through (F), and bound (B) fractions were analyzed using western blot. Ubiquitin recombinant antibody (80992-1-RR) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (SA00001-2) were used in the western blot analysis.$

Conjugation

Agarose, Magnetic Agarose
-_- / -_-

Cat No. uta, utak, utma, utmak
$The ubiquitin-trap agarose (uta) was used to immunoprecipitate endogenous ubiquitin and ubiquitinylated proteins from human (HEK293T), mouse (C2C12), and hamster (CHO) cell lines treated with MG-132. For each IP, samples of the input lysate (I), non-bound flow-through (F), and bound (B) fractions were analyzed using western blot. Ubiquitin recombinant antibody (80992-1-RR) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (SA00001-2) were used in the western blot analysis.$

Ubiquitin-Traps (4 total products)
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HA-Traps

1 Publications

Reviews (4)

$The HA-Trap Agarose (left) and a competitor resin (right) were used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. Immunoprecipitation with HA-Trap Agarose results in clean, single-band pulldowns without any heavy and light chain contamination. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.$

Conjugation

Agarose, Magnetic Agarose, Magnetic Particles M-270
-_- / -_-

Cat No. ata, atak, atd, atdk, atma, atmak
$The HA-Trap Agarose (left) and a competitor resin (right) were used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. Immunoprecipitation with HA-Trap Agarose results in clean, single-band pulldowns without any heavy and light chain contamination. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.$

HA-Traps (6 total products)
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His Fab-Trap® Agarose

$IP using His Fab-Trap® Agarose (hfa) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).$
View More Images (4)

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IP using His Fab-Trap® Agarose (hfa) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).
$IP using His Fab-Trap® Agarose (hfa) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).$

Application

IP, Co-IP

Conjugate

Agarose

Specificity

6*His, His-Tag (sequence: HHHHHH)

Sizes
-_- / -_-

Cat No. hfa
$IP using His Fab-Trap® Agarose (hfa) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).$

His Fab-Trap® Agarose
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

$IP using His Fab-Trap® Agarose (hfa) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).$

IP using His Fab-Trap® Agarose (hfa) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).

$His Fab-Trap® Agarose (hfa) was used for the Co-IP of AurKinB-His fusion protein and interacting partners from untransfected (mock) and transfected HEK293T cells. Multiplexed WB was done on Input (I), Flow-Through (F), and Bound (B) fractions of the IP. Detection was completed using His-tag Monoclonal Antibody (66005-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit for Mouse IgG (KFA023), CDC37 Monoclonal Antibody (66420-1-Ig) labeled with FLexAble CoraLite Plus 750 Kit for Mouse IgG2a (KFA044), and HSP90AB1 Monoclonal Antibody (67450-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit for Mouse IgG2b (KFA061).$

His Fab-Trap® Agarose (hfa) was used for the Co-IP of AurKinB-His fusion protein and interacting partners from untransfected (mock) and transfected HEK293T cells. Multiplexed WB was done on Input (I), Flow-Through (F), and Bound (B) fractions of the IP. Detection was completed using His-tag Monoclonal Antibody (66005-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit for Mouse IgG (KFA023), CDC37 Monoclonal Antibody (66420-1-Ig) labeled with FLexAble CoraLite Plus 750 Kit for Mouse IgG2a (KFA044), and HSP90AB1 Monoclonal Antibody (67450-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit for Mouse IgG2b (KFA061).

IP of GFP-His fusion protein from transfected HEK293T cells using His Fab-Trap Agarose (hfa) followed by a two-step elution using His-Peptide (hp). I: Input, F: Flow-through, E1: 1st elution, E2: 2nd elution, R: Residual.

IP of His-TOM70 and TOM70-His proteins using either His Fab-Trap Agarose (left) or a competitor resin product (right) from transfected HEK293T cells. The His Fab-Trap pulls down the TOM70 proteins more efficiently and with less background when compared to the competitor.
His Fab-Trap® Agarose Kit

$IP using His Fab-Trap® Agarose (hfa) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).$
View More Images (3)

×
IP using His Fab-Trap® Agarose (hfa) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).
$IP using His Fab-Trap® Agarose (hfa) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).$

Application

IP, Co-IP

Conjugate

Agarose

Specificity

6*His, His-Tag (sequence: HHHHHH)

Sizes
-_- / -_-

Cat No. hfak
$IP using His Fab-Trap® Agarose (hfa) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).$

His Fab-Trap® Agarose Kit
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

$IP using His Fab-Trap® Agarose (hfa) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).$

IP using His Fab-Trap® Agarose (hfa) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).

$His Fab-Trap® Agarose (hfa) was used for the Co-IP of AurKinB-His fusion protein and interacting partners from untransfected (mock) and transfected HEK293T cells. Multiplexed WB was done on Input (I), Flow-Through (F), and Bound (B) fractions of the IP. Detection was completed using His-tag Monoclonal Antibody (66005-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit for Mouse IgG (KFA023), CDC37 Monoclonal Antibody (66420-1-Ig) labeled with FLexAble CoraLite Plus 750 Kit for Mouse IgG2a (KFA044), and HSP90AB1 Monoclonal Antibody (67450-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit for Mouse IgG2b (KFA061).$

His Fab-Trap® Agarose (hfa) was used for the Co-IP of AurKinB-His fusion protein and interacting partners from untransfected (mock) and transfected HEK293T cells. Multiplexed WB was done on Input (I), Flow-Through (F), and Bound (B) fractions of the IP. Detection was completed using His-tag Monoclonal Antibody (66005-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit for Mouse IgG (KFA023), CDC37 Monoclonal Antibody (66420-1-Ig) labeled with FLexAble CoraLite Plus 750 Kit for Mouse IgG2a (KFA044), and HSP90AB1 Monoclonal Antibody (67450-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit for Mouse IgG2b (KFA061).

IP of His-TOM70 and TOM70-His proteins using either His Fab-Trap Agarose (left) or a competitor resin product (right) from transfected HEK293T cells. The His Fab-Trap pulls down the TOM70 proteins more efficiently and with less background when compared to the competitor.
His Fab-Trap® Magnetic Agarose

$IP using His Fab-Trap® Magnetic Agarose (hfma) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).$
View More Images (3)

×
IP using His Fab-Trap® Magnetic Agarose (hfma) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).
$IP using His Fab-Trap® Magnetic Agarose (hfma) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).$

Application

IP, Co-IP

Conjugate

Magnetic Agarose

Specificity

6*His, His-Tag (sequence: HHHHHH)

Sizes
-_- / -_-

Cat No. hfma
$IP using His Fab-Trap® Magnetic Agarose (hfma) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).$

His Fab-Trap® Magnetic Agarose
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

$IP using His Fab-Trap® Magnetic Agarose (hfma) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).$

IP using His Fab-Trap® Magnetic Agarose (hfma) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).

$His Fab-Trap® Magnetic Agarose (hfma) was used for the Co-IP of AurKinB-His fusion protein and interacting partners from untransfected (mock) and transfected HEK293T cells. Multiplexed WB was done on Input (I), Flow-Through (F), and Bound (B) fractions of the IP. Detection was completed using His-tag Monoclonal Antibody (66005-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit for Mouse IgG (KFA023), CDC37 Monoclonal Antibody (66420-1-Ig) labeled with FLexAble CoraLite Plus 750 Kit for Mouse IgG2a (KFA044), and HSP90AB1 Monoclonal Antibody (67450-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit for Mouse IgG2b (KFA061).$

His Fab-Trap® Magnetic Agarose (hfma) was used for the Co-IP of AurKinB-His fusion protein and interacting partners from untransfected (mock) and transfected HEK293T cells. Multiplexed WB was done on Input (I), Flow-Through (F), and Bound (B) fractions of the IP. Detection was completed using His-tag Monoclonal Antibody (66005-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit for Mouse IgG (KFA023), CDC37 Monoclonal Antibody (66420-1-Ig) labeled with FLexAble CoraLite Plus 750 Kit for Mouse IgG2a (KFA044), and HSP90AB1 Monoclonal Antibody (67450-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit for Mouse IgG2b (KFA061).

IP of GFP-His fusion protein from transfected HEK293T cell using His Fab-Trap® Magnetic Agarose (hfma) followed by a two-step elution with His-Peptide (hp). I: Input, F: Flow-Through, E1: 1st elution, E2: 2nd elution, R: Residual.

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