Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using KHC0004 (Insulin IHC Kit)

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using KHC0004 (Insulin IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using KHC0004 (Insulin IHC Kit).

Rat pancreas tissue were subjected to SDS PAGE followed by western blot with 28609-1-AP (insulin1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 28609-1-AP (insulin1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 28609-1-AP (insulin1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Rat pancreas tissue were subjected to SDS PAGE followed by western blot with 28588-1-AP (Insulin1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28588-1-AP (Insulin1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28588-1-AP (Insulin1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

insulin1 fusion protein Ag29988 SDS-PAGE

Purity of recombinant human IGF-I was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human IGF-I (Cat no: HZ-1322) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant IGF-I for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-14 ng/mL.

Proteintech IGF-I (Cat no: HZ-1322) demonstrates equivalent fold induction and a 2-fold decrease in EC50 compared to leading competitors. Recombinant human IGF-I stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant IGF-I for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-14 ng/mL.

Purity of recombinant human IL-12 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP recombinant human IGF-I (Cat no: HZ-1322-GMP) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant IGF-I for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 2-14 ng/mL.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-IGF2BP2 (IP:11601-1-AP, 3ug; Detection:11601-1-AP 1:500) with HEK-293 cells lysate 1000ug.

Various lysates were subjected to SDS PAGE followed by western blot with 11601-1-AP (IGF2BP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.