Various lysates were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP result of anti-GAD65 (IP:20746-1-AP, 4ug; Detection:20746-1-AP 1:1000) with mouse brain tissue lysate 3440ug.
Various lysates were subjected to SDS PAGE followed by western blot with 21760-1-AP (GAD65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP result of anti-GAD65 (IP:21760-1-AP, 3ug; Detection:21760-1-AP 1:300) with rat brain tissue lysate 4000ug.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21760-1-AP (GAD65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAD65 antibody (21760-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using GAD65 antibody (21760-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
