1x10^6 mouse bone marrow cells were surface stained with 0.25 ug FITC Plus Anti-Mouse CD11b (M1/70) (FITC-65055, Clone:M1/70), and 0.25 ug APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (APC-65140, Clone: RB6-8C5), and 0.25 ug FITC Plus Rat IgG2b Isotype Control (LTF-2) (FITC-65211, Clone: LTF-2). Cells were not fixed.
1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human CD73 Mouse Recombinant Antibody (CL488-65564, Clone:AD2), or CoraLite® Plus 488 Mouse IgG2a Isotype Control (CL488-65208, Clone: C1.18.4). Cells were not fixed. Lymphocytes were gated.
1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.5 ug FITC Plus Anti-Mouse CD80 (B7-1) (16-10A1) (FITC-65076, Clone: 16-10A1) (red), or 0.5 ug FITC Armenian Hamster IgG Isotype Control (PIP) (FITC-65210, Clone: PIP) (blue). Cells were not fixed.
1X10^6 PHA-stimulated (5 ug/ml, 3 days) human peripheral blood lymphocytes were surface stained with 5 ul APC Anti-Human PD-L1 (B7-H1) (APC-65081, Clone: 29E.2A3) (blue) or isotype control antibody (black). Cells were not fixed.
1X10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.125 ug FITC Plus Anti-Mouse CD86 (FITC-65068, Clone: GL1). Cells were not fixed.
1X10^6 LPS treated mouse splenocytes were surface stained with 0.125 ug FITC Plus Anti-Mouse CD86 (FITC-65068, Clone: GL1) (red) or 0.125 ug FITC-rat IgG2a isotype control. Cells were not fixed.
1X10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD86 (PE-65068, Clone: GL1). Cells were not fixed.
1X10^6 untreated (dashed line) or LPS-treated mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD86 (PE-65068, Clone: GL1) (red), or 0.2 ug Isotype Control (blue). Cells were not fixed.
1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD16/32 (65057-1-Ig, Clone: 93) and FITC anti-rat IgG2a Antibody at dilution 1:100, or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.
1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) and 0.5 ug FITC Anti-Mouse CD3ε (FITC-65060, Clone:145-2C11) or 0.5 ug FITC Armenian Hamster IgG Isotype Control (PIP). Cells were not fixed.
1X10^6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and 0.5 ug FITC Plus Anti-Mouse CD4 (FITC-65104, Clone: GK1.5) or 0.5 ug FITC Plus rat IgG2b isotype control. Cells were not fixed.
1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65187-1-Ig, Clone:Y1/82A) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD68 antibody (65187-1-Ig, Clone: Y1/82A ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31 antibody (65058-1-Ig, Clone: 390 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rat IgG(H+L).
1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD31 (65058-1-Ig, Clone: 390) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100 (blue). Cells were not fixed.
1X10^6 mouse bone marrow cells were surface co-stained with APC Anti-Mouse CD11b and 0.5 ug Anti-Mouse Ly-6G/Ly-6C (Gr-1) (65140-1-Ig, Clone:RB6-8C5) and FITC anti-Rat IgG2b Antibody at dilution 1:1000, or 0.5 ug Rat IgG2b isotype control. Cells were not fixed.
1X10^6 untreated (dashed lines) or LPS-treated (red) mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD86 (APC-65068, Clone:GL1) (red), or 0.2 ug Isotype Control (blue). Cells were not fixed.
1X10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD86 (APC-65068, Clone:GL1). Cells were not fixed.
1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 488 Anti-Mouse CD3 and 0.2 ug APC Anti-Mouse CD8a (APC-65069, Clone:53-6.7) or 0.2 ug Isotype Control. Cells were not fixed.
1X10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and 1 ug FITC Plus Anti-Mouse CD3 (FITC-65077, Clone: 17A2) or 1 ug isotype control. Cells were not fixed.
1X10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.5 ug PE Anti-Mouse CD80 (B7-1) (PE-65076, Clone:16-10A1) (red), or 0.5 ug Isotype Control. Cells were not fixed.
1X10^6 unstimulated or PMA and Ionomycin stimulated (in the presence of Brefeldin A) C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with 0.5 ug PE Anti-Mouse IFN gamma (PE-65153, Clone: XMG1.2).
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a / LAMP1 (65051-1-Ig, Clone:H4A3) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
1x10^6 C57BL/c mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Foxp3 (3G3) (65089-1-Ig, Clone: 3G3) or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21), FITC anti-Mouse IgG1 Antibody, and CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 C57BL/6 mouse bone marrow cells were surface co-stained with FITC Plus Anti-Mouse Ly-6G/Ly-6C (Gr-1) and 0.1 ug APC Anti-Mouse CD11b (APC-65055, Clone: M1/70) or 0.1 ug APC Rat IgG2b Isotype Control (LTF-2) (APC-65211, Clone: LTF-2). Cells were not fixed.
100 ul human peripheral blood were surface stained with 20.00 ul FITC Anti-Human CD34 (FITC-65183, Clone: QBEnd-10) and then treated with red blood cell lysis buffer. Cells were not fixed.