1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human MYH9 (11128-1-AP) and Fluorescein (FITC)–conjugated Goat Anti-Rabbit IgG(H+L) (SA00003-2) at dilution 1:200 (red), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.
1X10^6 HeLa cells were stained with 0.2 ug Lamin B1 antibody (66095-1-Ig, red) and Fluorescein (FITC)–conjugated Goat Anti-Mouse IgG(H+L) (SA00003-1) with dilution 1:100. Control antibody (blue). Cell were fixed with 90% MeOH.
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:100 and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) SA00003-11. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:100 and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) SA00003-11. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107b (65052-1-Ig, Clone:ABL-93) and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) (SA00003-11) at dilution 1:50 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH.
Immunofluorescent analysis of A431 cells, using Calnexin antibody 10427-2-AP at 1:50 dilution and SA00003-8 Fluorescein (FITC)–conjugated Donkey Anti-Rabbit IgG(H+L).
Human IgG (0.2 ug) were subjected to SDS PAGE followed by western blot with SA00003-12 at dilution of 1:1000 incubated at room temperature for 1.5 hours.
