Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD20 (Human IgG1) labeled with FlexAble FITC Plus Kit (KFA109) and anti-human CD19 (Mouse IgG1, clone 4G7) conjugated with PE (PE-65197).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 antibody (clone HI30, 65109-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD4 antibody (clone RPA-T4, 65143-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble FITC Plus Kit (KFA048).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble FITC Plus Kit (KFA048).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble FITC Plus Kit (KFA068). Cells are not fixed, lymphocytes are gated.
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA066).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble FITC Plus Kit (KFA068).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble CoraLite Plus 750 Kit (KFA064).
Flow cytometry of Jurkat cells. 1X10^6 Jurkat cells were intracellularly stained with anti-GAPDH antibody (10494-1-AP) labeled with FlexAble FITC Plus Kit (KFA008, red) or with isotype control antibody labeled with FlexAble FITC Plus Kit (KFA008, cyan).
Flow cytometry of Jurkat cells. 1X10^6 Jurkat cells were intracellularly stained with anti-HSP90 antibody (13171-1-AP) labeled with FlexAble FITC Plus Kit (KFA008, red) or with isotype control antibody labeled with FlexAble FITC Plus Kit (KFA008, cyan).
Flow cytometry of Jurkat cells. 1X10^6 Jurkat cells were intracellularly stained with anti-p62/SQSTM1 antibody (18420-1-AP) labeled with FlexAble FITC Plus Kit (KFA008, red) or with isotype control antibody labeled with FlexAble FITC Plus Kit (KFA008, cyan).
Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right).
Cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble FITC Plus for Rat Kappa Light Chain (KFA126 ) and CD3 antibody (CL750-65151).
1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble FITC Plus Kit (KFA126) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite Plus 647 Kit (KFA123). Cells were not fixed.
1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1, red) or rat IgG2a isotype control (65209-1-Ig, blue) labeled with FlexAble FITC Plus for Rat Kappa Light Chain (KFA126). Cells were not fixed.
