Retinal organoids (day 60) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and Cytokeratin 19 using 10712-1-AP at 1:200 (red). Nuclear stain DAPI (blue). Scale bar = 100 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

Various lysates were subjected to SDS PAGE followed by western blot with 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 19 (10712-1-AP) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

WB result of KRT19 antibody (60187-1-IG, 1:10,000) with si-Control and si-KRT19 transfected HeLa cells.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 19 (60187-1-Ig, Clone:3G1E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using KHC0033 (Cytokeratin 19 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0033 (Cytokeratin 19 IHC Kit)

Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using KHC0033 (Cytokeratin 19 IHC Kit).

MCF7 cells were subjected to SDS PAGE followed by western blot with 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

WB result of KRT19 antibody (14965-1-AP, 1:50,000) with si-Control and si-KRT19 transfected HeLa cells.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14965-1-AP (Cytokeratin 19 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cytokeratin 19 antibody (14965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 MCF-7 cells were stained with 0.2 ug Anti-Human Cytokeratin 19 (14965-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-60187 (Cytokeratin 19 antibody) at dilution of 1:50.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Cytokeratin 19 (CL488-60187, Clone:3G1E4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Adult mouse small intestine stained for Cytokeratin 19 (green, CL488-10712) and BMI1 (red, 10832-1-AP). BMI1 stains intestinal stem cells located at the base of the villi. As these stem cells differentiate into intestinal epithelial cells, they acquire Cytokeratin 19 expression. Cytokeratin 19 was conjugated to Coralite 488. Samples were fixed in 4% PFA, embedded in paraffin, and imaged on a confocal microscope. Image credit: @Immunofluorescence on Instagram.

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®488 Cytokeratin 19 antibody (CL488-14965) at dilution of 1:100.

1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Cytokeratin 19 (CL488-14965) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Cytokeratin 19 (CL647-60187, Clone:3G1E4) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Cytokeratin 19 (CL555-60187, Clone:3G1E4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.