1X10^6 HepG2 cells were stained with 0.2 ug Anti-Human MYH9 (11128-1-AP) and Cy3–conjugated Goat Anti-Rabbit IgG(H+L) (SA00009-2) at dilution 1:100 (red) Control Antibody in black. Cells were fixed with 4% PFA with 0.1 trition.
1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD45 (65082-1-Ig, Clone: 2D1) and Cy3–conjugated Goat Anti-Mouse IgG(H+L) (SA00009-1) at dilution 1:50 (green), and 0.5 ug Control Antibody. Cells were not fixed.