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human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (25682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (25682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD63 antibody (25682-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (66009-1-Ig, Clone: 2D4H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
A549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000.
IP result of anti-Beta Actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug.
Immunohistochemical analysis of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens).
Various lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Beta Actin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Beta Actin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using Beta Actin antibody (66009-1-Ig, Clone: 2D4H5 ) at dilution of 1:3000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Beta Actin (66009-1-Ig, Clone:2D4H5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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Untreated and MG132 treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Untreated and MG132 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Untreated and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human NRF2, NFE2L2 (80593-1-RR, Clone:1I21) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IP result of anti-NRF2, NFE2L2 (IP:80593-1-RR, 4ug; Detection:80593-1-RR 1:700) with HeLa cells lysate 1520 ug.
Immunohistochemical analysis of paraffin-embedded Brown disease slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80593-1-RR (NRF2, NFE2L2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using NRF2, NFE2L2 antibody (80593-1-RR, Clone: 1I21 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Biolayer interferometry (BLl) kinetic assays of 80593-1-RR against Human NRF2, NFE2L2 were performed. The affinity constant is 0.191 nM.
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Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP result of anti-GAPDH (IP:10494-1-AP, 3ug; Detection:10494-1-AP 1:3000) with A549 cells lysate 3500ug.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH antibody (10494-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).
1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug GAPDH Polyclonal antibody (10494-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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Various lysates were subjected to SDS PAGE followed by western blot with 50599-2-Ig (BAX antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB result of BAX antibody (50599-2-Ig, 1:5000) with si-Control and si-BAX transfected HeLa cells.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 50599-2-Ig (BAX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 50599-2-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-BAX (IP:50599-2-Ig, 4ug; Detection:50599-2-Ig 1:1000) with Raji cells lysate 3160 ug.
1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human BAX (50599-2-Ig) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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A431 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-human BCL2 (IP:12789-1-AP, 4ug; Detection:12789-1-AP 1:1000) with HL-60 cells lysate 4000 ug.
WB result of BCL2 (12789-1-AP, 1:1000) with si-Control and si-BCL2 transfected Jurkat cells.
1x10^6 HeLa cells were intracellularly stained with 0.8 ug human BCL2 Polyclonal antibody (12789-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using human BCL2 antibody (12789-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (human BCL2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (human BCL2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
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Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP result of anti-NRF2, NFE2L2 (IP:16396-1-AP, 4ug; Detection:16396-1-AP 1:6000) with HeLa cells lysate 1520 ug.
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using NRF2, NFE2L2 antibody (16396-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
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Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP (HIF1a antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB result of HIF-1 alpha antibody (20960-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells.
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20960-1-AP (HIF-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20960-1-AP (HIF-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (20960-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IP result of anti-HIF-1 alpha (IP:20960-1-AP, 4ug; Detection:20960-1-AP 1:300) with HeLa cells lysate 4000ug.
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40X of FTLD-U case stained by 10782-2-AP and 60019-2-Ig, showing dystrophic neurites. (Figs were provided by Linda K. Kwong).
Various lysates were subjected to SDS PAGE followed by western blot with 10782-2-AP (TDP-43 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 (N-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 (N-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB result of TDP-43 antibody (10782-2-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TDP-43 antibody (10782-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IP result of anti-TDP-43 (IP:10782-2-AP, 4ug; Detection:10782-2-AP 1:50000) with HeLa cells lysate 1520 ug.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (10782-2-AP) at dilution of 1:6000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (10782-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
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WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.
Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP result of anti-NF-κB p65 (IP:10745-1-AP, 3ug; Detection:10745-1-AP 1:1000) with HeLa cells lysate 5000ug.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10745-1-AP (p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
Immunofluorescent analysis of (4% PFA) fixed TNF alpha treated HT-1080 cells using NF-κB p65 antibody (10745-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
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Various lysates were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone H3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17168-1-AP (Histone H3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17168-1-AP (Histone H3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-Histone-H3 (IP:17168-1-AP, 4ug; Detection:17168-1-AP 1:8000) with MCF-7 cells lysate 2120 ug.
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone H3 antibody (17168-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red).
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Histone H3 Polyclonal antibody (17168-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-Lamin B1 (IP:12987-1-AP, 4ug; Detection:12987-1-AP 1:20000) with HeLa cells lysate 1560 ug.
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Lamin B1 (12987-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 12987-1-AP (Lamin B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin B1 antibody (12987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
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HEK-293T cells and transfected HEK-293T lysates were subjected to SDS PAGE followed by western blot with 20543-1-AP (DYKDDDDK tag antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
IP result of anti-DYKDDDDK tag (IP:20543-1-AP, 4ug; Detection:20543-1-AP 1:10000) with Transfected HEK-293 cells lysate 400 ug.
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Various lysates were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB result of P53 antibody (10442-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells.
Various lysates were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP result of anti-P53 (IP:10442-1-AP, 4ug; Detection:10442-1-AP 1:1000) with A431 cells lysate 3000ug.
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10442-1-AP (P53 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (10442-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (10442-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 66005-1-Ig (6*His, His-Tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated Goat Anti-Mouse IgG(H+L).
Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (66005-1-Ig) at various dilutions.
IP result of anti-6*His, His-Tag (IP:66005-1-Ig, 7ug; Detection:66005-1-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug.
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 6*His, His-Tag antibody (66005-1-Ig, Clone: 1B7G5 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
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Western blot of HA-tagged fusion protein with anti-HA-tag (51064-2-AP) at various dilutions.
IP result of anti-HA tag (IP:51064-2-AP, 3ug; Detection:51064-2-AP 1:2000) with Transfected HEK-293 cells lysate 500ug.
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 51064-2-AP (HA tag antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of Transfected HEK-293 cells using 51064-2-AP (HA tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
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WB result of Beta Catenin antibody (51067-2-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Catenin transfected HeLa cells.
Various lysates were subjected to SDS PAGE followed by western blot with 51067-2-AP (Beta Catenin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Beta Catenin antibody (51067-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP result of anti-Beta Catenin (IP:51067-2-AP, 4ug; Detection:51067-2-AP 1:500) with mouse liver tissue lysate 6400ug.
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Beta Catenin antibody (51067-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human Beta Catenin (51067-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (51067-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).
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Western blot of eGFP-tagged fusion protein with anti-eGFP-tag (50430-2-AP) at various dilutions.
IP result of anti-GFP tag (IP:50430-2-AP, 4ug; Detection:50430-2-AP 1:10000) with Transfected HEK-293T cells lysate 400 ug.
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 50430-2-AP (GFP tag Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using GFP tag antibody (50430-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag antibody (50430-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
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WB result of c-MYC antibody (10828-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-MYC transfected A549 cells.
Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP result of anti-c-MYC (IP:10828-1-AP, 4ug; Detection:10828-1-AP 1:1000) with MCF-7 cells lysate 1660 ug.
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10828-1-AP (c-MYC antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human c-MYC (10828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
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Jurkat cells (25 μg/lane) were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human PARP1 (13371-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
Various lysates were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Anti-Fas treated HeLa cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
IP result of anti-PARP1 (IP:13371-1-AP, 4ug; Detection:13371-1-AP 1:600) with K-562 cells lysate 5000ug.
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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WB result of CHOP antibody (15204-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHOP transfected HeLa cells.
Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using CHOP; GADD153 antibody (15204-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
1X10^6 HeLa cells untreated (dashed lines) or treated with Tunicamycin (20 μg/mL, 4 h; red) were intracellularly stained with 0.4 ug Anti-Human CHOP; GADD153 (15204-1-AP) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
IP result of anti-CHOP; GADD153 (IP:15204-1-AP, 4ug; Detection:15204-1-AP 1:4000) with C6 cells lysate 1600 ug.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).