Various lysates were subjected to SDS PAGE followed by western blot with 21652-1-AP (Cardiac Troponin I antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21652-1-AP (Cardiac Troponin I antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21652-1-AP (Cardiac Troponin I antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin I antibody (21652-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

WB result of Cardiac Troponin I antibody (21652-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cardiac Troponin I transfected HEK-293 cells.

IP result of anti-Cardiac Troponin I (IP:21652-1-AP, 4ug; Detection:21652-1-AP 1:30000) with mouse heart tissue lysate 1200 ug.

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin I antibody (21652-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using KHC0327 (TNNI3 IHC Kit).

Phosphatase inhibitor treated mouse heart tissue, λ phosphatase treated mouse heart tissue, and non-treated mouse heart tissue were subjected to SDS PAGE followed by western blot with 30937-1-AP (Phospho-TNNI3 (Ser23/24) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.