The cell population was analyzed in THE SSC/FSC diagram:<br>FITC-A was selected on the X axis to denote CL488-AnnexinV. The Y-axis is represented by PE-A.<br><br>The meanings of the regions in the figure above:<br>Q1-UL (CL488-AnnexinV)-/PI+, cells in this region are necrotic. There may also be a small number of late apoptotic cells in it, even those with mechanical damage.<br>Q1-UR :(cl488-annexinv)+/PI+, cells in this region are late apoptotic cells.<br>Q1-LR :(cl488-annexinv)+/PI-, cells in this region are early apoptotic cells.<br>Q1-LL :(cl488-annexinv)-/PI-, cells in this region are living cells.<br><br>The apoptosis rate was usually calculated using Q1-UR+Q1-LR, late apoptosis + early apoptosis group (i.e., all AnnexinV-positive groups).

Green: Staining with CL488-Annexin V for apooptotic cells or early apoptotic cells;<br>Red: Staining with PI for dead cells;<br>Yellow: double staining with CL488-Annexin V and PI for necrotic cell or late apoptotic cells.

The above experimental results are based on Jurkat cells cultured for three days to perform a flow cytometry experiment to detect the apoptotic cells in the cell sample.

Staurosporine treated HeLa cells for 4.5 h.

Immunofluorescent analysis of (4% PFA) fixed A549 cells using the CoraLite® Plus 488-conjugated Phalloidin antibody, CL488-Phalloidin, at dilution of 1:400. The nuclei were counterstained with DAPI.

NIH3T3 mouse fibroblasts. 1:50 dilution of CoraLite® Plus 488-Phalloidin. Nuclei are stained with Hoechst 33342. Scale bar is 10 um. Images taken by Katarzyna Szymanksa-DeWijs from Hannover Medical School.

Q2-UL：Cell Group with Calcein AM -, EthD-I + (dead cells)； <br>Q2-LL：Cell Group with Calcein AM-, EthD-I - (cell debris)； <br>Q2-LR：Cell Group with Calcein AM +, EthD-I - (live cells).

The above experimental results are based on Jurkat cells cultured for three days to perform a flow cytometry experiment to detect the apoptotic cells in the cell sample.

Staurosporine treated HeLa cells for 4 h.

Mouse thymocytes cells were heat killed at 65℃ for 10 minutes and then mixed with live mouse thymocytes cells. Cells were then stained with Phantom Dye Red 780 Viability Dye. Viable gate is indicated.

Cell Line：HeLa cell line <br>Medium：DMEM+10%FBS <br>Culture Conditions：37℃，5%CO2，add CCK-8 for 2h, 450nm R2=0.9953<br>

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®594-conjugated Phalloidin antibody, CL594-Phalloidin, at dilution of 1:400. The nuclei were counterstained with DAPI.

Mouse thymocytes cells were heat killed at 65℃ for 10 minutes and then mixed with live mouse thymocytes cells. Cells were then stained with Phantom Dye Blue 516 Viability Dye. Viable gate is indicated.