Purity of recombinant human Transferrin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human Transferrin (Cat no: HZ-1317) stimulates dose-dependent proliferation of the OKT4 mouse hybridoma cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. OKT4 cells were treated with increasing concentrations of recombinant Transferrin for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 75-400 ng/mL.
 

Purity of GMP recombinant human Transferrin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant GMP human Transferrin (Cat no: HZ-1317-GMP) stimulates dose-dependent proliferation of the OKT4 mouse hybridoma cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. OKT4 cells were treated with increasing concentrations of recombinant Transferrin for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 75-400 ng/mL.

human urine lysates were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 40x lens).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 17435-1-AP (Transferrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17435-1-AP (Transferrin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using KHC0377 (TF IHC Kit).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using KHC0377 (TF IHC Kit).

Various lysates were subjected to SDS PAGE followed by western blot with 30337-1-AP (TF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

TF fusion protein Ag11668 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 10084-2-AP (CD71 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10084-2-AP (CD71 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10084-2-AP (CD71 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-CD71 (IP:10084-2-AP, 4ug; Detection:10084-2-AP 1:500) with HeLa cells lysate 2400ug.

Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (10084-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (10084-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human CD71 Mouse IgG2a Recombinant Antibody (65605-1-MR, Clone: OKT9) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD71 Mouse IgG2a Recombinant Antibody (65605-1-MR, Clone: OKT9) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (black). Cells were not fixed.

1X10^6 C57BL/6 mouse bone marrow cells were surface co-stained with APC Anti-Mouse TER-119 (TER-119) and 0.5 ug Anti-Mouse CD71 (65289-1-Ig, Clone: RI7217) or 0.5 ug Rat IgG2a Isotype Control (2A3), and FITC anti-Rat IgG2a Antibody at dilution 1:100. Cells were not fixed.

Various lysates were subjected to SDS PAGE followed by western blot with 17691-1-AP (SH3BP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-SH3BP4 (IP:17691-1-AP, 4ug; Detection:17691-1-AP 1:300) with mouse lung tissue lysate 3600ug.

Immunofluorescent analysis of HeLa cells using 17691-1-AP (SH3BP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 65236-1-Ig (CD71 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 65236-1-Ig (CD71 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 65236-1-Ig (CD71 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 30574-1-AP (CD71 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30574-1-AP (CD71 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30574-1-AP (CD71 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-CD71 (IP:30574-1-AP, 4ug; Detection:30574-1-AP 1:10000) with HeLa cells lysate 1320 ug.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD71 antibody (30574-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD71 antibody (30574-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).