Immunofluorescence analysis of human pluripotent stem cell-derived astrocytes with S100β (15146-1-AP) at 1/200 (Magenta) and neurons with TUJ1 (66375-1-Ig) at 1:500 (Green). The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.3% Triton X-100. Alexa Fluor 488-conjugated goat anti-mouse IgG (1/500) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1/500) were used as the secondary antibodies. Nuclei were counterstained with DAPI (blue).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using S100 Beta antibody (15146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 15146-1-AP (S100 Beta antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using S100 Beta antibody (15146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 15146-1-AP (S100 beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 15146-1-AP (S100 Beta antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 A375 cells were intracellularly stained with 0.5 ug Anti-Human S100 Beta (15146-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

rat brain tissue were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:200 (under 40x lens).

Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using S100 Beta antibody (66616-1-Ig, Clone: 1G10F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0050 (S100B IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using KHC0050 (S100B IHC Kit).

human heart tissue were subjected to SDS PAGE followed by western blot with 16027-1-AP (S100A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 10x lens)..

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 40x lens)..

Immunofluorescent analysis of (4% PFA) fixed human ovary cancer cells using 16027-1-AP (S100A1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human ovary cancer cells using 16027-1-AP (S100A1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Standard curve of Human S100B ELISA Kit KE00103.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 S100 Beta antibody (CL488-15146) at dilution of 1:100.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 S100 Beta antibody (CL488-15146) at dilution of 1:100.

1X10^6 A375 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human S100 Beta (CL488-15146) (red), or 0.4 ug CoraLite®488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CoraLite®594 S100 Beta antibody (CL594-15146) at dilution of 1:100.

Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CoraLite®594 S100 Beta antibody (CL594-15146) at dilution of 1:100.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using KHC0248 (S100A1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0248 (S100A1 IHC Kit).

rat brain tissue were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using S100 Beta antibody (66616-1-Ig, Clone: 1G10F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.