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Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 25805-1-AP (NF-M antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Various lysates were subjected to SDS PAGE followed by western blot with 25805-1-AP (NF-M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 25805-1-AP (NEFM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M (25805-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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NF-M Monoclonal antibodies (2E3B12)
(Parent antibody plus 3 derived formats/conjugates)
- Reviews (1)
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Host/IsoType
Mouse/IgG1
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Reactivity
human, mouse, rat
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Applications
ELISA, FC (Intra), IF-P, IHC, WB
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Conjugation
CoraLite® Plus 488, CoraLite®594, Unconjugated
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CloneNo.
2E3B12
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Unconjugated parent antibody
Cat No. 66396-1-IgOther conjugate/format antibodies
Cat No. CL594-66396, 66396-1-PBS, CL488-66396
NF-M Monoclonal antibodies (2E3B12) (4 total products)-_- / -_-
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× The search results gallery shows a selection of our total image library
More validation images available on the product page
mouse brain and rat brain tissues were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB result of 20664-1-AP.
IP result of anti-NF-M-Specific (IP:20664-1-AP, 4ug; Detection:20664-1-AP 1:1000) with rat brain tissue lysate 4000ug.
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20664-1-AP (NF-M-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M-Specific (20664-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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× The search results gallery shows a selection of our total image library
More validation images available on the product page
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0045 (NF-M IHC Kit).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC0045 (NF-M IHC Kit).
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using KHC0045 (NF-M IHC Kit).
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using KHC0045 (NF-M IHC Kit).
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NF-M Monoclonal antibodies (2E3B12) (4 total products)
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