Mouse heart lysates were subjected to SDS PAGE followed by western blot with 17283-1-AP (MYL7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17283-1-AP (MYL7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Result of anti-MYL7 (IP:17283-1-AP, 4ug; Detection:17283-1-AP 1:700) with mouse heart tissue lysate 4000ug.
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL7 antibody (17283-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL7 antibody (17283-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 C2C12 cells were intracellularly stained with 0.5 ug Anti-Human MYL7 (17283-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).