Various lysates were subjected to SDS PAGE followed by western blot with 30388-1-AP (GPX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 30388-1-AP (GPX4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GPX4 (30388-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat testis tissue using GPX4 antibody (30388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat testis tissue using GPX4 antibody (30388-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WB result of GPX4 antibody (30388-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 transfected HEK-293 cells.

IP result of anti-GPX4 (IP:30388-1-AP, 4ug; Detection:30388-1-AP 1:3000) with rat testis tissue lysate 1880 ug.

A375 cells were subjected to SDS PAGE followed by western blot with 55027-1-AP (CSPG4,NG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 31623-1-AP (CSPG4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SMCP fusion protein Ag4984 SDS-PAGE