Mouse/Rat PDGF-BB ELISA Kit

Sensitivity

28.9 pg/mL

Range

62.5-4000 pg/mL

Reactivity

Mouse/Rat

Cat no : KE10034

Synonyms

PDGF B, PDGF BB, Pdgfb, PDGF-B, PDGFBB, PDGF-BB



Product Information

KE10034 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse/Rat PDGF-BB ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat PDGF-BB. The assay recognizes mouse/rat PDGF-BB. An antibody specific for mouse/rat PDGF-BB has been pre-coated onto the microwells. The mouse/rat PDGF-BB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat PDGF-BB is added to detect the captured mouse/rat PDGF-BB protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse/Rat PDGF-BB ELISA Kit
Tests 1 X 96 well plate
Sample type Mouse serum, Mouse plasma, Rat serum, Rat plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 28.9 pg/mL
Range 62.5-4000 pg/mL
Reactivity Mouse/Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 18591

Recovery

Sample TypeAverageRange
Mouse serum94%77%-115%
Rat serum102%76%-119%
Cell culture supernatants96%71%-117%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1202,153.169.73.2
220485.214.73.0
320135.24.02.9

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,958.937.21.9
224548.427.04.9
324163.57.14.4

Background Information

Platelet-derived growth factor (PDGF) belongs to the family of growth factors consisting of four secreted extracellular ligands including PDGF-A, -B, -C and -D. These isoforms, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, act via two receptor tyrosine kinases, PDGF receptors alpha and beta. PDGF isoforms stimulate growth, survival and motility of mesenchymal cells and certain other cell type. They have important functions during embryonal development and in the control of tissue homeostasis in the adult. PDGF-BB is one of the most recently studied isoforms and it plays an important role in skeletal development. PDGF-BB stimulates cell-mediated collagen gel contraction. PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms PDGF B, PDGF BB, Pdgfb, PDGF-B, PDGFBB, PDGF-BB

Publications

SpeciesSample TypeTitle
mousecell culture supernatants

Int J Biol Sci

Accelerated Bone Regeneration by Astragaloside IV through Stimulating the Coupling of Osteogenesis and Angiogenesis.

Authors - Feng Wang
mousecell culture supernatants

Front Pharmacol

M2 Macrophages Promote PDGFRβ+ Pericytes Migration After Spinal Cord Injury in Mice via PDGFB/PDGFRβ Pathway.

Authors - Ziyu Li
mousecell culture supernatants

Inflamm Regen

Imatinib inhibits pericyte-fibroblast transition and inflammation and promotes axon regeneration by blocking the PDGF-BB/PDGFRβ pathway in spinal cord injury

Authors - Fei Yao
mouseserum

Metabolism

Hepatocyte Ninjurin2 promotes hepatic stellate cell activation and liver fibrosis through the IGF1R/EGR1/PDGF-BB signaling pathway

Authors - Yifan Wang
humancell culture supernatants

Biomolecules

Novel Peptides with Dual Properties for Treating Pseudomonas aeruginosa Keratitis: Antibacterial and Corneal Wound Healing

Authors - Floriana Cappiello