IP sample detected with different secondary antibodies. Normal Mouse IgG (B900620) as control.

BLI binding kinetics of a monoclonal rabbit IgG anti-DYKDDDDK-tag antibody to a DYKDDDDK-tagged protein. A monoclonal rabbit IgG anti-DYKDDDDK-tag antibody was immobilized using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of a DYKDDDDK-tagged protein.

ELISA capture of a rabbit IgG antibody using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated. 50 nM Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Rabbit IgG antibody were titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

BLI binding kinetics of a polyclonal rabbit IgG anti-GFP antibody to eGFP. A polyclonal rabbit IgG anti-GFP antibody (ChromoTek GFP antibody [PABG1]) was immobilized using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of eGFP (ChromoTek EGFP, recombinant protein).

BLI binding kinetics of different human IgG antibodies. Different monoclonal human IgG antibodies were immobilized using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors. All four human IgG subclasses are stably captured by the Nano-CaptureLigand with negligible dissociation.

Immobilization of the Nano-CaptureLigand (green) on Biosensor (white) for biolayer interferometry (BLI) followed by capture of human IgG antibody (orange). The setup is ready for characterization of human IgG antibodies’ binding kinetics and affinity to substrate (red), human IgG antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma.

ELISA capture of human IgG1 lambda antibody using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated. 50 nM Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Human IgG1 lambda antibody was titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

Sandwich ELISA: Immobilization of the Nano-CaptureLigand on streptavidin coated microplates for ELISA. No biotinylation of the antibody is required. Coating of the streptavidin microplate with Nano-CaptureLigand (top left). Site-directed antibody immobilization via Nano-CaptureLigand (top right). Addition of sample including target (red, bottom left). Addition of detection and secondary antibody for read-out (bottom right).

Species reactivity of Secondary VHH.

Various lysates were subjected to SDS-PAGE followed by western blot with U2AF2 mouse monoclonal antibody (68166-1-Ig) at 1:20000. Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM001 were used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig) at dilution of 1:50000. Three batches of Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM001) were used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with EFTUD2 mouse monoclonal antibody (67855-1-Ig, isotype IgG1) at dilution of 1:10000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were both used at 0.05μg/mL for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with Flag tag mouse monoclonal antibody (sigma M2, isotype IgG1) at dilution of 1:50000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were both used at 0.05μg/mL for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with Beta actin mouse monoclonal antibody (60008-1-Ig, isotype IgM) at dilution of 1:50000. RGAM001 (left) and competitor’s non cross-adsorbed HRP-Goat anti-mouse (H+L) secondary antibody (right) were both used at 0.05μg/mL for detection.

Mouse total IgG, Mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgA monoclonal antibodies, Rat total IgG, Pig total IgG, Human total IgG, Bovine total IgG were coated at 100 ng/well. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM001) was used for detection. The result indicates that RGAM001 strongly binds to all Mouse IgGs, Mouse IgM and IgA as well as Rat IgG. It shows weak reactivity for pig IgG and does not react with other species tested in the experiment. 

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-PSMB1 polyclonal antibody (11749-1-AP) at dilution of 1:10000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-Beta Catenin polyclonal antibody (51067-2-AP) at dilution of 1:50000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-TOM70 polyclonal antibody (14528-1-AP) at dilution of 1:10000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-TDP43 recombinant antibody (80001-1-RR) at dilution of 1:20000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used at 1:20000 for detection.

Rabbit total IgG, Mouse total IgG, Rat total IgG, Pig total IgG, Human total IgG, Bovine total IgG were coated at 100 ng/well. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used for detection. The result indicates that RGAM001 is highly specific for rabbit IgG and does not react with other species tested in the experiment.

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, orange) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 555-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR003, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, orange) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 555-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR003, 1:500) and Multi-rAbCoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, red) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, orange) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, green). Multi-rAb CoraLite® Plus 555 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR003, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, red) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, green). Multi-rAb CoraLite® Plus 594 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR004, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (66630-1-Ig, Clone: 1B9G12 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-2-Ig. Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM002 was used at 1:500 for detection.

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, red). Multi-rAb CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM004, 1:500) .

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, green) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, orange). Multi-rAb CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 555 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM003, 1:500) .

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Myosin Light Chain 2/MLC-2V antibody (10906-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Icam-1 antibody (30324-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, red). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, magenta). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, 1:500) were used for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, green) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, orange). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 555-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM003, 1:500) were used for detection.

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-HLA class I rabbit polyclonal antibody (15240-1-AP) and Rabbit IgG Isotype Control 30000-0-AP. Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) RGAR002 was used at 1:500 for detection.

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, green) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, red). Multi-rAb CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM004, 1:500) .

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, green) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, red). Multi-rAb CoraLite® Plus 488 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM004, 1:500) .

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, red). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, green) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, red). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-2-Ig. Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM004 was used at 1:500 for detection.

Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, red) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

Immunofluorescence of Hela cells: Hela cells were fixed with 4% PFA and stained with Rabbit anti-Alpha Tubulin polyclonal antibody (11224-1-AP, 1:200, red) and mouse anti-NPM1 monoclonal antibody (60096-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.

Immunofluorescence of mouse cerebellum: mouse cerebellum FFPE section was stained with Rabbit anti-NeuN polyclonal antibody (26975-1-AP, 1:200, red) and mouse anti-Calbindin-D28k monoclonal antibody (66394-1-Ig, 1:200, green). Multi-rAb CoraLite® Plus 594 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR004, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

Immunofluorescence of rat brain: rat brain FFPE section was stained with Rabbit anti-GFAP polyclonal antibody (16825-1-AP, 1:200, red) and mouse anti-NeuN monoclonal antibody (66836-1-Ig, green). Multi-rAb CoraLite® Plus 594 conjugated Recombinant Goat anti-rabbit secondary antibody (RGAR004, 1:500) and Multi-rAb CoraLite® Plus 488 conjugated Goat Anti-Mouse Recombinant Secondary Antibody (H+L) were used for detection (RGAM002, 1:500) .

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

Immunofluorescent analysis of (4% PFA) fixed OCT-embedded frozen mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Band 3 / AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-HLA class I rabbit polyclonal antibody (15240-1-AP) and Rabbit IgG Isotype Control 30000-0-AP. Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) RGAR004 was used at 1:500 for detection.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Mouse Recombinant secondary antibody RGAM011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66065-2-Ig (CD31 mouse monoclonal antibody) at dilution of 1:10000 . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-mouse Recombinant secondary antibody RGAM011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66379-1-Ig (Beta Catenin mouse monoclonal antibody) at dilution of 1:20000 . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-mouse Recombinant secondary antibody RGAM011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using commercial IVD grade pan-CK mouse monoclonal antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-mouse Recombinant secondary antibody RGAM011 was used for detection.

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using other vendor's NeuN mouse monoclonal antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-mouse Recombinant secondary antibody RGAM011 was used for detection.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67479-1-Ig (SLC22A7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Mouse Recombinant secondary antibody RGAM011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Mouse Recombinant secondary antibody RGAM011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Mouse Recombinant secondary antibody RGAM011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80001-1-RR (TDP43 recombinant antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-Rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (smooth muscle actin recombinant antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (smooth muscle actin recombinant antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin polyclonal antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin polyclonal antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin polyclonal antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using commercial IVD grade KI67 antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using other vendor's NF-kappa B p65 rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using other vendor's BCL2 rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer. Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection. The staining was performed on Celnovte's CNT360-M1 autostainer.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using other vendor's ER rabbit recombinant antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Multi-rAb Polymer HRP-Goat anti-rabbit Recombinant secondary antibody RGAR011 was used for detection.

Live SKBR-3 cells immunostained with anti-HER2 (trastuzumab biosimilar) labeled with FlexAble CoraLite Plus 405 Antibody Labeling Kit for Human IgG (KFA108).   Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD20 (Human IgG1) labeled with FlexAble CoraLite® Plus 405 Kit (KFA108) and anti-human CD19 (Mouse IgG1, clone 4G7) conjugated with PE  (PE-65197).

Live A431 cells were immunostained with anti-EGFR (cetuximab biosimilar) labeled with FlexAble CoraLite Plus 488 Antibody Labeling Kit for Human IgG (KFA104). Nuclei are in blue.  Epifluorescence images were acquired with a 20x objective and post-processed.​

Live SKBR-3 cells immunostained with anti-HER2 (trastuzumab biosimilar) labeled with FlexAble CoraLite Plus 488 Antibody Labeling Kit for Human IgG (KFA104).  Nuclei are in blue.  Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD20 (Human IgG1) labeled with FlexAble CoraLite® Plus 488 Kit (KFA104) and anti-human CD19 (Mouse IgG1, clone 4G7) conjugated with PE  (PE-65197).

Immunofluorescence of A431 cells: PFA-fixed cells were stained with rat anti-Tubulin alpha antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green) and rat IgG1 kappa anti-RPA32 antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, red).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green) and rat IgG2b kappa anti-RNA PolymeraseII antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, red).  Epifluorescence images were acquired with a 20x objective and post-processed.

Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right). ​ Cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble CoraLite® Plus 488 for Rat Kappa Light Chain (KFA121) and CD3 antibody (CL750-65151).

1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1, red) or rat IgG2a isotype control (65209-1-Ig, blue) labeled with FlexAble CoraLite® Plus 488 for Rat Kappa Light Chain (KFA121). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 488 Kit (KFA121) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

Immunofluorescence of A431 cells: PFA-fixed cells were co-stained with rat anti-RNA Pol II Ser5 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta) and with rat anti-Tubulin alpha antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green). Cell nuclei were stained with DAPI (blue).   Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Live A431 cells were immunostained with anti-EGFR (cetuximab biosimilar) labeled with FlexAble CoraLite Plus 555 Antibody Labeling Kit for Human IgG (KFA105). Nuclei are in blue.​  Epifluorescence images were acquired with a 20x objective and post-processed.​

Live SKBR-3 cells immunostained with anti-HER2 (trastuzumab biosimilar) labeled with FlexAble CoraLite Plus 555 Antibody Labeling Kit for Human IgG (KFA105).  Nuclei are in blue.  Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD20 (Human IgG1) labeled with FlexAble CoraLite® Plus 555 Kit (KFA105) and anti-human CD19 (Mouse IgG1, clone 4G7) conjugated with APC (APC-65197).

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2b kappa anti-RNA Polymerase II antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, yellow) and rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, yellow) and rat IgG1 kappa anti-RPA32 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, red). Cell nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green) and rat IgG1 kappa anti-RPA32 antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, red).  Epifluorescence images were acquired with a 20x objective and post-processed.

Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right). ​ Cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble CoraLite® Plus 555 for Rat Kappa Light Chain (KFA122) and CD3 antibody (CL750-65151).

1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1, red) or rat IgG2a isotype control (65209-1-Ig, blue) labeled with FlexAble CoraLite® Plus 555 for Rat Kappa Light Chain (KFA122). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 555 Kit (KFA122) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green) and rat IgG2b kappa anti-RNA PolymeraseII antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, red).  Epifluorescence images were acquired with a 20x objective and post-processed.

Live A431 cells were immunostained with anti-EGFR (cetuximab biosimilar) and FlexAble CoraLite® Plus 594 Kit (KFA112, red). Nuclei are in cyan. This epifluorescence image was acquired at Zeiss Demo Center, Munich, with a 40x objective and post-processed.

Immunofluorescence of A431 cells: PFA-fixed cells were stained with anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite® Plus 594 Kit (KFA009, orange) and DAPI (blue). ​ Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of Hela cells. 1X10^6 Hela cells were intracellularly stained with anti-TOM20 rabbit polyclonal antibody (11802-1-AP, red) or with isotype control antibody (blue) labeled with FlexAble CoraLite® Plus 594 Kit (KFA009).

Flow cytometry of Hela cells. 1X10^6 Hela cells were intracellularly stained with anti-Mitofilin rabbit polyclonal antibody (10179-1-AP, red) or with isotype control antibody (blue) labeled with FlexAble CoraLite® Plus 594 Kit (KFA009).

Live A431 cells were immunostained with anti-EGFR (cetuximab biosimilar) labeled with FlexAble CoraLite Plus 647 Antibody Labeling Kit for Human IgG (KFA106). Nuclei are in blue.​  Epifluorescence images were acquired with a 20x objective and post-processed.​

Live SKBR-3 cells immunostained with anti-HER2 (trastuzumab biosimilar) labeled with FlexAble CoraLite Plus 647 Antibody Labeling Kit for Human IgG (KFA106).  Nuclei are in blue.  Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD20 (Human IgG1) labeled with FlexAble CoraLite® Plus 647 Kit (KFA106) and anti-human CD19 (Mouse IgG1, clone 4G7) conjugated with PE (PE-65197).

Immunofluorescence of A431 cells: PFA-fixed cells were stained with rat anti-RNA Pol II Ser5 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2b kappa anti-RNA Polymerase II antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, yellow) and rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, yellow) and rat IgG1 kappa anti-RPA32 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431 cells: PFA-fixed cells were co-stained with rat anti-RNA Pol II Ser5 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta) and with rat anti-Tubulin alpha antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green). Cell nuclei were stained with DAPI (blue).   Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescent analysis of FFPE mouse colon tissue slides with Rat anti-mouse Cdh1 antibody labeled with FlexAble CoraLite Plus 647 Kit (KFA123, magenta). With/without quencher. Cell nuclei were stained with DAPI (blue).

Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right). The cells were surface stained with CD3 antibody (CL488-65151) before fixation. Then, cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble CoraLite® Plus 647 kit (KFA123).

1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1) or rat IgG2a isotype control (65209-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed. Histogram, red: Rat IgG2a isotype control (65209-1-Ig); blue: Rat anti-mouse CD86 (65068-1-Ig).

1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite Plus 488 Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 647 (KFA123). Cells were not fixed.

Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right). ​ Cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble CoraLite® Plus 647 for Rat Kappa Light Chain (KFA123) and CD3 antibody (CL488-65151).

1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1, red) or rat IgG2a isotype control (65209-1-Ig, blue) labeled with FlexAble CoraLite® Plus 647 for Rat Kappa Light Chain (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 488 Kit (KFA121) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble FITC Plus Kit (KFA126) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 555 Kit (KFA122) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with rat anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.