Various lysates were subjected to SDS PAGE followed by western blot with 82248-1-RR (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP result of anti-FIS1(IP:82248-1-RR, 4ug; Detection:82248-1-RR 1:5000) with HeLa cells lysate 1720 ug.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82248-1-RR (FIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FIS1 antibody (82248-1-RR, Clone: 6N15 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FIS1 (82248-1-RR, Clone:6N15) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82248-1-RR (FIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).