Results

DYKDDDDK tag Monoclonal antibodies (8H6A10)

(Parent antibody plus 5 derived formats/conjugates)

361 Publications

Reviews (3)

Host/IsoType

Mouse/IgG2b

Reactivity

human, chicken, recombinant protein

Applications

ChIP, CoIP, FC (Intra), IF/ICC, IHC, IP, RIP, WB

Conjugation

CoraLite® Plus 647, CoraLite®594, FITC, HRP, Unconjugated

CloneNo.

8H6A10
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Unconjugated parent antibody
Cat No. 66008-4-Ig

Other conjugate/format antibodies
Cat No. CL594-66008, HRP-66008, FITC-66008, 66008-4-PBS, CL647-66008
DYKDDDDK tag Monoclonal antibodies (8H6A10) (6 total products)
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CoraLite® Plus 488-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope)

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Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66008-3 (DDDDK tag antibody), at dilution of 1:100.

Host/IsoType

Mouse/IgG2b

Reactivity

recombinant protein

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488

CloneNo.

1E7B4

Sizes
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Cat No. CL488-66008
CoraLite® Plus 488-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope)
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The search results gallery shows a selection of our total image library
More validation images available on the product page

Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66008-3 (DDDDK tag antibody), at dilution of 1:100.

1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope) (CL488-66008, Clone:1E7B4)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
DYKDDDDK tag Polyclonal antibodies

(Parent antibody plus 1 derived formats/conjugates)

1052 Publications

Reviews (20)

Host/IsoType

Rabbit/IgG

Reactivity

human, recombinant protein

Applications

ChIP, CoIP, IF/ICC, IHC, IP, RIP, WB

Conjugation

CoraLite® Plus 750, Unconjugated
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Unconjugated parent antibody
Cat No. 20543-1-AP

Other conjugate/format antibodies
Cat No. CL750-20543
DYKDDDDK tag Polyclonal antibodies (2 total products)
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DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)

1 Publications

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TDP-43-flag Transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 80801-2-RR (Ag2329 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

CloneNo.

240568A11

Sizes
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Cat No. 80801-2-RR
DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)
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The search results gallery shows a selection of our total image library
More validation images available on the product page

TDP-43-flag Transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 80801-2-RR (Ag2329 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IP result of anti-DYKDDDDK tag (IP:80801-2-RR, 4ug; Detection:80801-2-RR 1:3000) with Transfected HEK-293 cells lysate 400 ug.

1x10^6 HEK-293T transfected with TDP-43-flag were intracellularly stained with 0.4 ug DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope) (80801-2-RR, Clone:240568A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293T cells using DYKDDDDK tag antibody (80801-2-RR, Clone: 240568A11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Biolayer interferometry (BLl) kinetic assays of 80801-2-RR against DYKDDDDK tag were performed. The affinity constant is 15.3 pM.
DYKDDDDK-Fab-Traps

37 Publications

Reviews (8)

Applications

ChIP, CoIP, IP, RIP

Conjugation

Agarose, Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
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Cat No. ffa, ffap, ffak
DYKDDDDK-Fab-Traps (3 total products)
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3xDYKDDDDK-peptide

1 Publications

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DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

Application

Elution

Conjugate

Unconjugated

Sizes
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Cat No. fp
3xDYKDDDDK-peptide
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The search results gallery shows a selection of our total image library
More validation images available on the product page

DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.
Biotin-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

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Ag2329 (DYKDDDDK tagged protein) was coated onto microtiter plates at 0.073 µg/well and then incubated with a dilution series of Biotin-66008-4 (start concentration 500 ng/ml). And then detected with Streptavidin Poly-HRP (1:2000) followed by incubation with HRP Substrate, terminated with 2M H2SO4, then measuring the resulting absorbance at 450 nm. Negative control is 66008-4-Ig.

Host/IsoType

Mouse/IgG2b

Reactivity

recombinant protein

Applications

IF/ICC, ELISA

Conjugate

Biotin

CloneNo.

8H6A10

Sizes
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Cat No. Biotin-66008-4
Biotin-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)
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The search results gallery shows a selection of our total image library
More validation images available on the product page

Ag2329 (DYKDDDDK tagged protein) was coated onto microtiter plates at 0.073 µg/well and then incubated with a dilution series of Biotin-66008-4 (start concentration 500 ng/ml). And then detected with Streptavidin Poly-HRP (1:2000) followed by incubation with HRP Substrate, terminated with 2M H2SO4, then measuring the resulting absorbance at 450 nm. Negative control is 66008-4-Ig.

Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using Biotin DYKDDDDK tag antibody (Biotin-66008-4, Clone: 8H6A10 ) at dilution of 1:600 and Alexa Fluor 488 Streptavidin.
Recombinant DYKDDDDK tag protein (Contains same sequence as FLAG® Tag)

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Species

N/A

Source

E. coli-derived, PGEX-4T

Tag

GST

Format

Powder

Sizes
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Cat No. Ag2329
Recombinant DYKDDDDK tag protein (Contains same sequence as FLAG® Tag)
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The search results gallery shows a selection of our total image library
More validation images available on the product page

DYKDDDDK tag Ag2329 SDS-PAGE
DYKDDDDK tag Monoclonal antibodies (2B3C4)

(Parent antibody plus 1 derived formats/conjugates)

203 Publications

Reviews (7)

Host/IsoType

Mouse/IgG1

Reactivity

human, recombinant protein

Applications

CoIP, ELISA, IF/ICC, IHC, IP, RIP, WB

Conjugation

Unconjugated

CloneNo.

2B3C4
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Unconjugated parent antibody
Cat No. 66008-3-Ig

Other conjugate/format antibodies
Cat No. 66008-3-PBS
DYKDDDDK tag Monoclonal antibodies (2B3C4) (2 total products)
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DYKDDDDK tag Monoclonal antibodies (8H6A10)

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CoraLite® Plus 488-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope)

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DYKDDDDK tag Polyclonal antibodies

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DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)

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DYKDDDDK-Fab-Traps

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3xDYKDDDDK-peptide

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Biotin-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

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Recombinant DYKDDDDK tag protein (Contains same sequence as FLAG® Tag)

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DYKDDDDK tag Monoclonal antibodies (2B3C4)

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