1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Caveolin-1 (CL594-66067, Clone:6C2B2) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).