The cell population was analyzed in THE SSC/FSC diagram:<br>FITC-A was selected on the X axis to denote CL488-AnnexinV. The Y-axis is represented by PE-A.<br><br>The meanings of the regions in the figure above:<br>Q1-UL (CL488-AnnexinV)-/PI+, cells in this region are necrotic. There may also be a small number of late apoptotic cells in it, even those with mechanical damage.<br>Q1-UR :(cl488-annexinv)+/PI+, cells in this region are late apoptotic cells.<br>Q1-LR :(cl488-annexinv)+/PI-, cells in this region are early apoptotic cells.<br>Q1-LL :(cl488-annexinv)-/PI-, cells in this region are living cells.<br><br>The apoptosis rate was usually calculated using Q1-UR+Q1-LR, late apoptosis + early apoptosis group (i.e., all AnnexinV-positive groups).
Green: Staining with CL488-Annexin V for apooptotic cells or early apoptotic cells;<br>Red: Staining with PI for dead cells;<br>Yellow: double staining with CL488-Annexin V and PI for necrotic cell or late apoptotic cells.
1X10^6 mouse splenocytes were surface stained with CoraLite488-conjugated Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5) and then fixed with 1X Transcription Factor Fix/Perm buffer (PF00011-A) and permeabilized with 1X Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with CoraLite647-conjugated mouse IgG1 isotype control or 5 ul CoraLite647-conjugated Anti-Mouse Foxp3 (CL647-65089, Clone: 3G3).<br>