Untreated or Con A-treated (3 ug/ml, 72h) mouse splenocytes were subjected to SDS PAGE followed by western blot with 30648-1-AP (CTLA-4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Purity of Recombinant Human CTLA-4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.
Immobilized Human CD86 (Myc tag, His tag) at 0.5 μg/mL (100 μL/well) can bind Human CTLA-4 (hFc tag) with a linear range of 5-22 ng/mL.
