Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11242-1-AP (COXIV antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COXIV rabbit pAb (11242-1-AP) at dilution of 1:100 and alpha Tubulin mouse mAb (66031-1-Ig) at dilution of 1:200, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 11242-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66031-1-Ig.
Various lysates were subjected to SDS PAGE followed by western blot with 11242-1-AP (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11242-1-AP (COXIV antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human COXIV (11242-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.
IP Result of anti-COXIV (IP:11242-1-AP, 3ug; Detection:11242-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3500ug.