Various lysates were subjected to SDS PAGE followed by western blot with 28579-1-AP (CDA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDA (28579-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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HeLa cells were subjected to SDS PAGE followed by western blot with 17597-1-AP (CDAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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IP result of anti-CDAN1 (IP:17597-1-AP, 4ug; Detection:17597-1-AP 1:500) with K-562 cells lysate 3200ug.

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Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17597-1-AP (CDAN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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