Mo7e cells were subjected to SDS PAGE followed by western blot with 67135-2-Ig (CD33 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD33 (65272-1-Ig, Clone:WM-53) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed .
1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD33 (AB-65272, Clone: WM53) (red) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD33 (CR-65272, Clone: WM53) (red) or unstained. Cells were not fixed. Monocytes were gated.
Mo7e cells were subjected to SDS PAGE followed by western blot with 67135-2-Ig (CD33 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67135-2-PBS in a different storage buffer formulation.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD33 (CL405-65272, Clone: WM53) (red) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD33 (CL488-65272, Clone: WM53) (red) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD33 (CL555-65272, Clone: WM53) (red) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD33 (CL647-65272, Clone: WM53) (red) or unstained. Cells were not fixed. Monocytes were gated.
