Various lysates were subjected to SDS PAGE followed by western blot with 10600-1-AP (CD24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10600-1-AP (CD24 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10600-1-AP (CD24 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Various lysates were subjected to SDS PAGE followed by western blot with 67627-1-Ig (CD24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human CD24 (67627-1-Ig, Clone:1H5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD24 (M1/69) (65121-1-Ig, Clone: M1/69) or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were not fixed.
1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.06 ug APC-Rat IgG2b isotype control (left) or 0.06 ug APC Anti-Mouse CD24 (APC-65121, Clone: M1/69) (right). Cells were not fixed.
Various lysates were subjected to SDS PAGE followed by western blot with 67627-1-Ig (CD24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67627-1-PBS in a different storage buffer formulation.
1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human CD24 (67627-1-Ig, Clone:1H5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67627-1-PBS in a different storage buffer formulation.
1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD24 (CL488-65121, Clone:M1/69) (red), or 0.5 ug Control Antibody. Cells were not fixed .
1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.5 ug CoraLite® Plus 647-conjugated Anti-Mouse CD24 (CL647-65121, Clone: M1/69). Cells were not fixed.
1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and CoraLite® Plus 647-conjugated rat IgG2b isotype control antibody. Cells were not fixed.
1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 750 Anti-Mouse CD24 (CL750-65121, Clone:M1/69) (red), or 0.5 ug Isotype Control. Cells were not fixed.
1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug CoraLite®594 Anti-Mouse CD24 (CL594-65121, Clone:M1/69) (red), or 0.5 ug Isotype Control. Cells were not fixed.