WB result of C9orf72 antibody (25757-1-AP, 1:250 incubated at RT for 1 hour) with si-C9orf72 HEK293 cells, C9orf72 CRISPR KO HEK293 cells; normal HEK293 cells and non-targeting control (NTC) siRNA transfected HEK293 cells as control. The ~50 kDa is the C9orf72 Long isoform. (Data from Dr Chris Webster, Postdoc in Kurt de Vos’s group at SITRAN).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25757-1-AP (C9orf72 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25757-1-AP (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
mouse brain tissue were subjected to SDS PAGE followed by western blot with 25757-1-AP (C9orf72 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 25757-1-AP (C9orf72 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).