Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 14676-1-AP (ATP5A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14676-1-AP (ATP5A1 antibody) at dilution of 1:200 (under 10x lens).
Various lysates were subjected to SDS PAGE followed by western blot with 14676-1-AP (ATP5A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP result of anti-ATP5A1 (IP:14676-1-AP, 4ug; Detection:14676-1-AP 1:15000) with HeLa cells lysate 1750 ug.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14676-1-AP (ATP5A1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14676-1-AP (ATP5A1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATP5A1 antibody (14676-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5A1 antibody (14676-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ATP5A1 Monoclonal antibodies (1B10H3)
(Parent antibody plus 4 derived formats/conjugates)
KO/KD Validated
24 Publications
Reviews (2)
Host/IsoType
Mouse/IgG2b
Reactivity
human, mouse, rat, hamster, monkey
Applications
ELISA, FC (Intra), IF/ICC, IHC, IP, WB
Conjugation
CoraLite® Plus 488, CoraLite®555, CoraLite®594, Unconjugated