Various lysates were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67943-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG16L1 antibody (67943-1-Ig, Clone: 1G5G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67943-1-PBS in a different storage buffer formulation.